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Delignification and swelling of Eucalpytus wood ahead of enzymatic hydrolysis of the cellulosic fraction


, : Delignification and swelling of Eucalpytus wood ahead of enzymatic hydrolysis of the cellulosic fraction. Process biochemistry0(6): 537-545

An effective process for the chemical-biotechnological utilization of Eucalyptus globulus wood is reported. In a first stage, a treatment with catalyzed acetic acid solutions allowed the solubilization of both lignin and hemicelluloses, leaving cellulose as solid residue. A subsequent swelling treatment with NH4OH solutions caused structural modifications to the cellulosic fraction, giving a substrate highly susceptible to the enzymic hydrolysis. A centered, incomplete, factorial design of experiments was carried out in order to establish the inter-relationships between three selected operational variables (NH4OH concentration used in the chemical treatment and both liquid/solid and enzyme/substrate ratios employed in the hydrolysis step) on the kinetics and yields of enzymatic hydrolysis. From experimental data, generalized kinetic models giving the influence of the operational variables on the hydrolysis stage were developed. Further discussion on relevant aspects involved in the prosess is provided.

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Accession: 015451427

DOI: 10.1016/0032-9592(94)00063-8

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Related references

Parajo, J.C.; Alonso, J.L.; Santos, V., 1995: Delignification and swelling of Eucalyptus wood ahead of enzymatic hydrolysis of the cellulosic fraction. An effective process for the chemical-biotechnological utilization of Eucalyptus globulus wood is reported. In the first stage, a treatment with catalysed acetic acid solutions allowed the solubilization of both lignin and hemicelluloses, leaving...

Sudo, K.; Matsumura, Y.; Shimizu, K., 1976: Enzymatic hydrolysis of wood. I. Effect of delignification on hydrolysis of woods by Trichoderma viride cellulase. Holocelluloses delignified to various degrees were prepared from two hardwoods and two softwoods by means of acid sodium chlorite, and were treated with a cellulolytic enzyme preparation from Trichoderma viride. The extent of enzymic hydrolysis wa...

Teleman, A., 1995: NMR structural studies of wood hemicelluloses during delignification and cellooligosaccharides during enzymatic hydrolysis. This paper was presented to the XVII National NMR symposium.

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