Section 16
Chapter 15,470

Determination of aflatoxins by reversed-phase high-performance liquid chromatography with post-column in-line photochemical derivatization and fluorescence detection

Joshua, H.

Journal of Chromatography A 654(2): 247-254


ISSN/ISBN: 0021-9673
DOI: 10.1016/0021-9673(93)83367-2
Accession: 015469591

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Post-column in-line photochemical derivatization permits fluorescence detection of all four common aflatoxins B1, B2, G1 and G2. Chromatographic evidence indicates that the photolysis causes the hydration of the non-fluorescent B1 and G1 components to the B2a and G2a components respectively. Analysis of naturally contaminated corn samples show no interfering peaks and permits the determination of 1 and 0.25 ppb (w/w) of B1 and B2, respectively.

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