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Determination of organic acids in grape musts, wines and vinegars by high-performance liquid chromatography


Determination of organic acids in grape musts, wines and vinegars by high-performance liquid chromatography



Journal of Chromatography A 655(1): 111-117



ISSN/ISBN: 0021-9673

DOI: 10.1016/0021-9673(93)87018-h

A method for the determination of tartaric, malic, shikimic, lactic, acetic, citric, succinic, citramalic and fumaric acid in musts, wines and vinegars is proposed, based on high-performance liquid chromatography using two 3-m C18 columns (250 × 4.6 mm I.D.) in series, providing an efficiency between 50 000 and 80 000 theoretical plates/m. The mobile phase is phosphate buffer (pH 2.35) to which is added a small amount of methanol (3%) as polar modifier. The relative standard deviations were <6% and no effect of sample preparation (filtration and passage through Sep-Pak C18) on the results was observed.

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Accession: 015473342

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