Digitized chromatographic fingerprint spectrum of water soluble components of Salvia miltiorrhiza Bunge by high speed countercurrent chromatography

Gu, M.; Wang, X.; Ouyang, F.

Journal of Liquid Chromatography and Related Technologies 30(18): 2789-2800

2007


ISSN/ISBN: 1082-6076
DOI: 10.1080/10826070701560975
Accession: 015514091

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Abstract
Water soluble components are amajor part of active components of Salvia miltiorrhiza Bunge, a very important species of TCM, whose quality control is valuable. Twelve water extracted crude samples of Salvia miltiorrhiza Bunge were analyzed and fingerprints of them were developed by high speed countercurrent chromatography (HSCCC). Three major components in each sample were identified. Their digitized chromatographic fingerprint spectrum (DCFS) were developed by an improved method with relative retention time, relative peak area, relative standard deviation of relative retention time, relative standard deviation of relative peak area, difference rate, and fingerprint characteristic. HSCCC-DCFS was a quantitative and intuitive method for the fingerprinting of water soluble components of Salvia miltiorrhiza Bunge.