EurekaMag.com logo
+ Translate

Direct cloning of differential expression genes with full-length by a modified solid subtractive hybridization


, : Direct cloning of differential expression genes with full-length by a modified solid subtractive hybridization. Journal of Beijing Forestry University 29(5): 67-72

Some differently expressed genes, including several full-length cDNAs were isolated and screened from cold-acclimated Ammopiptanthus mongolicus seedlings. Sequence analysis showed that the screened cDNAs shared homologies with previous reported genes involved in plant cold adaptation.

(PDF 0-2 workdays service)

Accession: 015516180

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Huang, X.; Yuan, Z.; Cao, X., 2002: Direct cloning of full-length cell differentially expressed genes by multiple rounds of subtractive hybridization based on long-distance PCR and magnetic beads. This article describes an infant with multiple congenital anomalies who underwent laparoscopic insertion of a gastrostomy tube. Prior repair of congenital heart defects had resulted in complete heart block necessitating the implantation of an epic...

Huang, H.; Wang, G.; Zhao, Y.; Shi, P.; Luo, H.; Yao, B., 2010: Direct and efficient cloning of full-length genes from environmental DNA by RT-qPCR and modified TAIL-PCR. Environmental DNA (eDNA) is defined as the total DNA that can be isolated from environmental samples. In total, therefore, eDNA includes a vast functional genes, and various approaches have been developed to retrieve full-length functional genes f...

Zhao, Z.; Huang, X.; Li, N.; Zhu, X.; Chen, S.; Cao, X., 1999: Direct cloning of cell differential expression genes with full-length by a new strategy based on the multiple rounds of 'long distance' polymerase chain reaction and magnetic beads mediated subtraction. The paper described a new cDNA subtractive cloning strategy. This strategy was based on the 'cap-finder' method, 'long distance' polymerase chain reaction (PCR), streptavidin magnetic beads mediated subtraction, and spin column...

Meiring, T.; Mulako, I.; Tuffin, M.I.; Meyer, Q.; Cowan, D.A., 2011: Retrieval of full-length functional genes using subtractive hybridization magnetic bead capture. Numerous gene-specific PCR methods have been developed for the cultivation-independent discovery of novel genes from complex environmental DNA samples. The recovery of full-length genes is, however, technically challenging. Here, we present an eff...

Wan, J.S.; Erlander, M.G., 1997: Cloning differentially expressed genes by using differential display and subtractive hybridization. Methods in Molecular Biology 85: 45-68

Rebrikov, D.V., 2008: Identification of Differential Genes by Suppression Subtractive Hybridization: II. Subtractive Hybridization. INTRODUCTIONSuppression subtractive hybridization (SSH) is one of the most powerful and popular methods for generating subtracted cDNA or genomic DNA libraries. This technique can be used to compare two mRNA populations and obtain cDNAs representi...

J.M.ng Chun; Chen Hong Ju; Yan Hua; Shen Hou Feng, 2003: Cloning and identification of differential genomic genes of antibiotic resistant Neisseria gonorrhoeae by suppression subtractive hybridization. Objective: To clone the differential genes in antibiotic resistence Neisseria gonorrhoeae. Methods: Using suppression subtractive hybridization construct, to construct the library which contains the differential genes between antibiotic resistance...

Nurminskaya, M.V.; Birk, D.E., 1998: Differential expression of genes associated with collagen fibril growth in the chicken tendon: identification of structural and regulatory genes by subtractive hybridization. Collagen fibril growth is a very rapid and abrupt process, resulting in a 4- to 5-fold increase in fibril length between 16 and 18 days of chicken metatarsal tendon development. This fibril growth is due to a postdepositional fusion/association of...

Bao, B.L.ng; Yang, G.M.i; Shi, Z.Y.; Ren, D.M.ng, 2006: Differential expression genes cloned in pro-metamorphosing Paralichthys olivaceus by suppression subtractive hybridization. To clone the differential expressional genes between the pre-metamorphosis (17 day post hatching, DPH) and pro-metamorphosis stage (23 DPH), we used suppression subtractive hybridization (SSH) to construct a cDNA library of pro-metamorphosis floun...

Rebrikov, D.V., 2008: Identification of Differential Genes by Suppression Subtractive Hybridization: VI. Differential Hybridization with Tester and Driver DNA Probes. INTRODUCTIONSuppression subtractive hybridization (SSH) is one of the most powerful and popular methods for generating subtracted cDNA or genomic DNA libraries. This technique can be used to compare two mRNA populations and obtain cDNAs representi...