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Direct control by calcium of 25-hydroxycholecalciferol-1-hydroxylase activity in chick kidney mitochondria






Biochemical and Biophysical Research Communications 54(2): 512-518

Direct control by calcium of 25-hydroxycholecalciferol-1-hydroxylase activity in chick kidney mitochondria

Kidney mitochondria were isolated from rachitic chicks and their activity in the metabolism of 25-OH-D3 was studied in relation to the amount of calcium added in vitro. The addition of 0.050.2 mM calcium to a mitochondrial suspension caused a marked and dose-related stimulation of 1-hydroxylation. A sharp decline in the activity was induced by higher concentrations (0.3-0.5 mM) of calcium. The rate of 24-hydroxylation was not influenced by calcium. In these effects, calcium was relatively specific among various divalent cations. These data strongly suggest that calcium is directly involved in the regulation of the vitamin D activation in kidney mitochondria.


Accession: 015516214

PMID: 4756783

DOI: 10.1016/0006-291x(73)91451-4



Related references

Bar, A.; Hurwitz, S.; Maoz, A., 1980: The 25-hydroxycholecalciferol-1-hydroxylase activity of chick kidney cells: direct effect of parathyroid. Febs Letters 113(2): 328-330

Ghazarian, J.G.; DeLuca, H.F., 1974: 25-Hydroxycholecalciferol-1-hydroxylase: a specific requirement for NADPH and a hemoprotein component in chick kidney mitochondria. Archives of Biochemistry and Biophysics 160(1): 63-72

Suda T.; Horiuchi N.; Sasaki S.; Ezawa I.; Ogata E., 1975: Importance of intra mitochondrial calcium for 25 hydroxy cholecalciferol 1 hydroxylase activity in chick kidney mitochondria. Talmage, Roy V , Maureen Owen And John A Parsons (Ed ) Excerpta Medica International Congress Series, No 346 Calcium-Regulating Hormones Proceedings Of The Fifth Parathyroid Conference, Oxford, England July 21-26, 1974 Xiii+486p Illus Excerpta Medica: Amsterdam, Netherlands (Dist in U S A And Canada By American Elsevier Publishing Company, Inc : New York, N Y ) Isbn 90-219-0280-X; Isbn 0-444-15161-3 411-414

Bar, A.; Dubrov, D.; Eizner, U.; Hurwitz, S., 1978: Calcium-binding protein and kidney 25-hydroxycholecalciferol-1-hydroxylase activity in turkey poults. A vitamin D-dependent calcium-binding protein (CaBP) has been found in the intestinal mucosa of turkeys (Meleagris gallopavo). This protein is similar, if not identical, to the corresponding one found in the intestine of the chicken (Gallus domest...

Horiuchi, N.; Suda, T.; Sasaki, S.; Ogata, E.; Ezawa, I., 1975: The regulatory role of calcium in 25-hydroxycholecalciferol metabolism in chick kidney in vitro. The regulatory effect of calcium added in vitro on 25-hydroxycholecalciferol metabolism was studied in kidney mitochondria and in renal tubules from vitamin D-deficient chicks. The addition of calcium (0.05 – 0.2 mm) to mitochondrial suspensions...

Fraser, D.R.; Kodicek, E., 1973: Regulation of 25-hydroxycholecalciferol-1-hydroxylase activity in kidney by parathyroid hormone. Chickens 35 days old, normally fed or depleted of vitamin D, were given by intracardial injection 0.5 mu g cholecalciferol-4-14C,1-3H and were killed 24 h later. The intestinal mucosa had 6 to 8 times as much 1,25-dihydroxycholecalciferol (1,25-DH...

Fenton, E.; Britton, H.G., 1980: 25-Hydroxycholecalciferol 1 alpha -hydroxylase activity in the kidney of the fetal, neonatal and adult guinea pig. Homogenates of foetal, newborn and maternal guineapig kidney and placenta were compared with homogenates of nonpregnant adult kidney for their ability to hydroxylate 25-hydroxycholecalciferol. The activity in the foetal kidney near term was simila...

Fenton, E.; Britton, H.G., 1980: 25-hydroxycholecalciferol 1 alpha-hydroxylase activity in the kidney of the fetal, neonatal and adult guinea pig. Homogenates of fetal, newborn and maternal kidney and placenta have been compared with homogenates of non-pregnant adult kidney for their ability to hydroxylate 25-hydroxycholecalciferol. The activity in the fetal kidney near term is similar to th...

Swaminathan, R.; Sommerville, B.A.; Care, A.D., 1977: The effect of dietary calcium on the activity of 25-hydroxycholecalciferol 1-hydroxylase and Ca absorption in vitamin D-replete chicks. The effect was studied of dietary Ca on the activity of 25-hydroxycholecalciferol 1-hydroxylase and on Ca absorption in vitamin D-replete chicks. Chicks 10 days old were given for 10 days diets differing in Ca contents with cholecalciferol 65 nmol...

Swaminathan, R.; Sommerville, B.A.; Care, A.D., 1977: The effect of dietary calcium on the activity of 25-hydroxycholecalciferol-1-hydroxylase and Ca absorption in vitamin D-replete chicks. 1. As most of the studies on the regulation of renal 25-hydroxycholecalciferol-1-hydroxylase (25-HCC-1-hydroxylase) activity have been done in marginally-vitamin D-deficient animals and as it is known that vitamin D administration suppresses the s...