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Direct control by calcium of 25-hydroxycholecalciferol-1-hydroxylase activity in chick kidney mitochondria






Biochemical and Biophysical Research Communications 54(2): 512-518

Direct control by calcium of 25-hydroxycholecalciferol-1-hydroxylase activity in chick kidney mitochondria

Kidney mitochondria were isolated from rachitic chicks and their activity in the metabolism of 25-OH-D3 was studied in relation to the amount of calcium added in vitro. The addition of 0.050.2 mM calcium to a mitochondrial suspension caused a marked and dose-related stimulation of 1-hydroxylation. A sharp decline in the activity was induced by higher concentrations (0.3-0.5 mM) of calcium. The rate of 24-hydroxylation was not influenced by calcium. In these effects, calcium was relatively specific among various divalent cations. These data strongly suggest that calcium is directly involved in the regulation of the vitamin D activation in kidney mitochondria.


Accession: 015516214

PMID: 4756783

DOI: 10.1016/0006-291x(73)91451-4



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