EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Direct in vivo access to potential gene targets of the RPD3 histone deactylase using fitness-based interferential genetics


Yeast 24(7): 575-587
Direct in vivo access to potential gene targets of the RPD3 histone deactylase using fitness-based interferential genetics
Using the fitness-based interferential genetics (FIG) approach in yeast, potential in vivo gene targets of the Rpd3 histone deacetylase were selected. In agreement with previous studies using different methods, three genes were found to be involved in the translational machinery (MRPL27, FHL1 and RDN1). Moreover, other selected genes are linked to cell-cycle control (CSE4, AMN1, VAC17 and GRR1). In addition to playing a crucial role in cell cycle progression to the S phase and participating in the G(2)-M transition, GRR1 has important functions related to nutrient import to the cell via the the derepression of hexose transporters and the induction of amino acid permeases. Consistent with this, FIG selection also retrieved: the PMA1 gene, encoding the plasma H+-membrane ATPase; FOL2 and FOL3, involved in folic acid biosynthesis; and UBR2, which indirectly downregulates the proteasome genes. Finally, the other selected genes, ISU1, involved in the biosynthesis of the iron-sulphur cluster in mitochondria, and the less well functionally defined BSC5 and YBR270c, may participate in the cell's antioxidant and stress defence. The genes emerging from this FIG selection thus appear to be part of the downstream molecular mechanisms of the TOR signalling pathway, accounting for its effects on cell proliferation and longevity. From our results on gene expression under conditions of RPD3 overexpression, and by comparison with the available pharmacogenomics studies, it is proposed that FIG could be an invaluable approach for contributing to our understanding of complex cell regulatory systems. Copyright (C) 2007 John Wiley & Sons, Ltd.


Accession: 015516583

PMID: 17533620

DOI: 10.1002/yea.1495



Related references

A fitness-based interferential genetics approach using hypertoxic/inactive gene alleles as references. Molecular Genetics and Genomics 281(4): 437-445, 2009

Raf60, a novel component of the Rpd3 histone deacetylase complex required for Rpd3 activity in Saccharomyces cerevisiae. Journal of Biological Chemistry 280(52): 42552-6, 2005

Histone deactylase gene expression profiles are associated with outcomes in blunt trauma patients. Journal of Trauma and Acute Care Surgery 80(1): 26-32; Discussion 32-3, 2016

Linking susceptibility to histone deactylase inhibitors and basal gene expression profiles in acute myeloid leukaemia. 2007

Histone deacetylase activity of Rpd3 is important for transcriptional repression in vivo. Genes & Development 12(6): 797-805, 1998

Cytoskeletal proteins regulate chromatin access of BR-C transcription factor and Rpd3-Sin3A histone deacetylase complex in Drosophila salivary glands. Nucleus 2(5): 489-499, 2012

The regulation of gene activity by histones and the histone deacetylase RPD3. Cold Spring Harbor Symposia on Quantitative Biology 63: 391-399, 1999

The Histone Deacetylase Gene Rpd3 Is Required for Starvation Stress Resistance. Plos One 11(12): E0167554-E0167554, 2016

Targeted recruitment of the Sin3-Rpd3 histone deacetylase complex generates a highly localized domain of repressed chromatin in vivo. Molecular and Cellular Biology 18(9): 5121-5127, 1998

Role of histone deacetylase Rpd3 in regulating rRNA gene transcription and nucleolar structure in yeast. Molecular and Cellular Biology 26(10): 3889-3901, 2006