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Direct regeneration from cotyledon culture of lycopersicon Esculentum Mill

Journal of Phytological Research 20(1): 67-70

Direct regeneration from cotyledon culture of lycopersicon Esculentum Mill

Cotyledonexplants of tomato (Lycopersicon esculentum Mill. cv. Pusaruby) cultured on MS medium supplemented with different auxins like IAA, NAA and 2,4-D produced callus whereas callusing was more pronounced in IAA followed by NAA, while 2,4,-D gave little response. Cytokinin alone produced callus with shoot primordia developed from cut edges. BAP alone showed better response in shoot differentiation than Kn. Among various combinations of auxins and cytokinins IAA+BAP and IAA + Kn produced green nodular callus with shoots whereas, NAA+Kn and NAA + BAP produced only callus. The percentage of responding cultures, and mean number of shoots per explant produced on IAA (0.5mg/l) + BAP (4.5 and 5.0 mg/l) were higher than all the concentrations and combinations of IAA+Kn used. However, maximum number of shoots were observed at 0.5 mg/l IAA + 5.0 mg/l BAR Microshoots were rooted on MS basal medium supplemented with 0.5 mg/I IAA.

Accession: 015516897

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