+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Early decompression of the injured optic nerve reduces axonal degeneration and improves functional outcome in the adult rat



Early decompression of the injured optic nerve reduces axonal degeneration and improves functional outcome in the adult rat



Experimental Brain Research 179(1): 121-130



The putative beneficial role of an early decompression of injured CNS tissue following trauma remains controversial. In this study, we approach this scientific query using a standardized injury of the optic nerve in adult rats. Adult Sprague-Dawley rats were subjected to a standardized optic nerve constriction injury by applying a loose ligature around the nerve for 5 min, 1, 6 or 24 h. All animals were sacrificed at 28 dpi. Viable axons distal to the injury were quantified using semithin sections, and regenerative fibers were studied using antisera to neurofilament and GAP43. Axonal degeneration and glial scar development were analyzed using Fluoro-Jade staining and anti-GFAP, respectively. Visual function was studied with visual evoked potentials (VEP). No significant differences were observed between 1 and 6 h of optic nerve compression. However, the number of viable axons analyzed with neurofilament and on semithin sections, decreased significantly between 6 and 24 h, paralleled by an increase in Fluoro-Jade labeled axonal debris (P < 0.001). GFAP-IR density was significantly higher (P < 0.001) in the 24 h compression group in comparison to 6 h. VEP showed preserved, but impaired visual function in animals subjected to compression up to 6 h, compared to an abolished cortical response at 24 h. Regenerative GAP43-positive sprouts were occasionally found distal to the lesion in animals subjected to compression up to 6 h, but not at 24 h. These findings suggest that early optic nerve decompression within hours after the initial trauma is beneficial for functional outcome.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 015559243

Download citation: RISBibTeXText

PMID: 17103208

DOI: 10.1007/s00221-006-0775-1


Related references

Inhibition of Fas-mediated apoptosis through administration of soluble Fas receptor improves functional outcome and reduces posttraumatic axonal degeneration after acute spinal cord injury. Journal of Neurotrauma 23(5): 604-616, 2006

Long-distance axonal regeneration induced by CNTF gene transfer is impaired by axonal misguidance in the injured adult optic nerve. Neurobiology of Disease 51: 202-213, 2013

βB2-Crystallin Promotes Axonal Regeneration in the Injured Optic Nerve in Adult Rats. Cell Transplantation 24(9): 1829-1844, 2015

Reaction of NG2 cells to axonal degeneration in the developing and adult rat optic nerve. Society for Neuroscience Abstracts 26(1-2): Abstract No -515 13, 2000

Use of a Y-tube conduit after facial nerve injury reduces collateral axonal branching at the lesion site but neither reduces polyinnervation of motor endplates nor improves functional recovery. Neurosurgery 70(6): 1544-56; Discussion 1556, 2012

Methylprednisolone treatment does not influence axonal regeneration or degeneration following optic nerve injury in the adult rat. Journal of Neuro-Ophthalmology 24(1): 11-18, 2004

Attenuation of Axonal Degeneration by Calcium Channel Inhibitors Improves Retinal Ganglion Cell Survival and Regeneration After Optic Nerve Crush. Molecular Neurobiology 54(1): 72-86, 2017

Retrograde myelin and axonal degeneration after optic nerve transection in adult frogs xenopus laevis. Anatomical Record 199(3): 30A, 1981

A temporal study of axonal degeneration and glial scar formation following a standardized crush injury of the optic nerve in the adult rat. Restorative Neurology and Neuroscience 22(1): 1-10, 2004

Combination treatment of experimental stroke with Niaspan and Simvastatin, reduces axonal damage and improves functional outcome. Journal of the Neurological Sciences 294(1-2): 107-111, 2010

Minocycline treatment reduces delayed oligodendrocyte death, attenuates axonal dieback, and improves functional outcome after spinal cord injury. Journal of Neuroscience 24(9): 2182-2190, 2004

Calcium channel inhibition-mediated axonal stabilization improves axonal regeneration after optic nerve crush. Neural Regeneration Research 11(8): 1245-1246, 2016

Long-term outcome of optic nerve encasement and optic nerve decompression in patients with fibrous dysplasia: risk factors for blindness and safety of observation. Neurosurgery 59(5): 1011-7; Discussion 1017-8, 2006

Non synaptic modulation of axonal conduction by gaba b receptors in the neonatal rat optic nerve baclofen improves high frequency impulse conduction in the premyelinated optic nerve. Society for Neuroscience Abstracts 17(1-2): 1169, 1991

Optic nerve sheath decompression for nonarteritic ischemic optic neuropathy improves multiple visual function measurements. Archives of Ophthalmology 109(5): 667-671, 1991