Effects and interactions of juvenile hormone and beta-ecdysone on Drosophila imaginal discs cultured in vitro

Chihara, C.J.; Fristrom, J.W.

Developmental Biology 35(1): 36-46


ISSN/ISBN: 0012-1606
PMID: 4207110
DOI: 10.1016/0012-1606(73)90005-5
Accession: 015647382

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Evagination of mass-isolated imaginal discs of Drosophila melanogaster is induced in vitro by β-ecdysone and reversibly inhibited by Cecropia juvenile hormone (JH). Both optically active enantiomers of the C18 Cecropia Jh inhibit ecdysone-induced evagination and produce developmental abnormalities when fed to larvae. The β-ecdysone-induced increase in incorporation into Rna is inhibited by JH. However, Jh fails to inhibit the ecdysone-stimulated increase in transport of 3H-uridine into disc cells. In fact, Jh itself induces an increase in uridine transport into discs. After incubation of discs with Jh and β-ecdysone for 3 hr and removal of the hormones, there is an immediate increase in incorporation into Rna similar to that induced by β-ecdysone itself. This result indicates that Jh does not prevent β-ecdysone from binding to its receptors, but prevents only the increase in incorporation into RNA. It is proposed that Jh may prevent the transcription of ecdysone-derepressed genes by acting as a corepressor.