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Expression of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) spike protein fragment (S144-643) and its immunogenicity



Expression of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) spike protein fragment (S144-643) and its immunogenicity



Journal of Huazhong Agricultural University 25(1): 1-5



The gene fragment of S144-643 was subcloned into the pET-28b plasmid and expressed in Escherichia coli. The S144-643 protein mainly existed in bacterial inclusion bodies. It was purified from bacterial inclusion bodies, and refolded with the refolding buffer containing reduced glutathione and oxidized glutathione. The SDS-PAGE showed the protein with a molecular mass of about 55 kDa.

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