Fractionation and identification of Euglena gracilis cytoplasmic and chloroplastic tRNAs and mapping of tRNA genes on chloroplast DNA
Kuntz, M.; Keller, M.; Crouse, E.J.; Burkard, G.; Weil, J.H.
Current Genetics 6(1): 63-69
ISSN/ISBN: 0172-8083 PMID: 24186373 DOI: 10.1007/bf00397643
The cytoplasmic and chloroplast tRNAs of Euglena gracilis Z strain were fractionated by two-dimensional gel electrophoresis and identified by aminoacylation. Purified chloroplast tRNAs, labeled in vitro with |(32)P|, were hybridized to endonuclease restriction fragments of chloroplast DNA, allowing the corresponding tRNA genes to be localized on the physical map of Euglena chloroplast DNA.