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Heterogeneity of mitochondrial DNA from Saccharomyces cerevisiae and genetic information for tRNA

, : Heterogeneity of mitochondrial DNA from Saccharomyces cerevisiae and genetic information for tRNA. Nucleic Acids Research 2(10): 1777-1786

Mitochondrial DNA from wild-type Saccharomyces cerevisiae and from an "extreme" petite mutant were analyzed by hybridization of several tRNAs on DNA fragments of different buoyant density, obtained by sonication and fractionation on a CsCl gradient. The hybridization patterns show that the genes for tRNAser, tRNAphe, tRNAhis, tRNAval, tRNAileu are present on wild-type mitochondrial DNA, while only genes for tRNAser and tRNAhis are present on petite mitochondrial DNA; moreover the hybridization patterns indicate that these genes are not clustered and suggest that more than one gene might exist for tRNAser and tRNAhis.

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Accession: 016005920

PMID: 1103084

DOI: 10.1093/nar/2.10.1777

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