+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Isolation and characterization of Viviparous-1 genes in wheat cultivars with distinct ABA sensitivity and pre-harvest sprouting tolerance



Isolation and characterization of Viviparous-1 genes in wheat cultivars with distinct ABA sensitivity and pre-harvest sprouting tolerance



Journal of Experimental Botany 58(11): 2863-2871



Pre-harvest sprouting (PHS) of wheat reduces the quality and economic value of grain, and increasing PHS tolerance is one of the most important traits in wheat breeding. Two new Vp-1B alleles related to PHS tolerance were identified on the 3BL chromosome of bread wheat and were designated Vp-1Bb and Vp-1Bc. Sequence analysis showed that Vp-1Bb has a 193 bp insertion and Vp-1Bc has a 83 bp deletion located in the third intron region of the Vp-1B gene, and that they shared 95.43% and 97.89% similarity, respectively, with the sequence of AJ400713 (Vp-1Ba) at the nucleotide level. Their sequences were deposited in the GenBank under the accession numbers DQ517493 and DQ517494. Semi-quantitative RT-PCR analysis showed that alternatively spliced transcripts of the Vp-1A, Vp-1B, and Vp-1D homologues were present and there were no differences in the splicing patterns or abundances of Vp-1A and Vp-1D from embryos 35 d after pollination between PHS-tolerant and -susceptible cultivars. Although Vp-1Ba, Vp-1Bb, and Vp-1Bc could each produce a set of transcripts, only one was correctly spliced and had the capacity to encode the full-length VP1 protein and was more highly expressed with Vp-1Bb and Vp-1Bc than with Vp-1Ba. Comparison of the expression patterns of Vp-1Ba, Vp-1Bb, and Vp-1Bc on different days after pollination also revealed that the expression of these genes was developmentally regulated. Furthermore, genotypes with different levels of tolerance to PHS respond differently to ABA exposure and differences in transcript levels of Vp-1Ba, Vp-1Bb, and Vp-1Bc were observed after ABA treatment. The results indicated that insertion or deletion in the third intron region might affect the expression of the Vp-1B gene and its sensitivity to ABA, and thus resistance to PHS.

(PDF emailed within 0-6 h: $19.90)

Accession: 016205683

Download citation: RISBibTeXText

PMID: 17630295

DOI: 10.1093/jxb/erm073


Related references

Expression of the aldehyde oxidase 3, ent-copalyl diphosphate synthase, and VIVIPAROUS 1 genes in wheat cultivars differing in their susceptibility to pre-harvest sprouting. Spanish Journal of Agricultural Research 15(1): e0701, 2017

Low temperature germinability and ABA sensitivity in wheat cultivars with pre-harvest sprouting tolerance. Wheat Information Service (98): 5-10, 2004

Exploiting the diversity of Viviparous-1 gene associated with pre-harvest sprouting tolerance in European wheat varieties. Euphytica 159(3): 411-417, 2008

Germination behavior of developing white wheat grains from cultivars varying in pre harvest sprouting tolerance. Plant Physiology 89(4 SUPPL): 168, 1989

Targeted Haplotype Comparisons between South African Wheat Cultivars Appear Predictive of Pre-harvest Sprouting Tolerance. Frontiers in Plant Science 9: 63, 2018

ABA sensitivity of developing embryos in pre-harvest sprouting resistant and susceptible wheat cultivars and the mechanism for their resistance. Journal of Triticeae Crops 25(2): 27-32, 2005

Development and validation of a Viviparous-1 STS marker for pre-harvest sprouting tolerance in Chinese wheats. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 115(7): 971-980, 2007

Cloning of seed dormancy genes (TaSdr) associated with tolerance to pre-harvest sprouting in common wheat and development of a functional marker. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 127(4): 855-866, 2014

Testing method of pre harvesting sprouting and genealogical pedigree of pre harvest sprouting resistant cultivars in wheat. Japanese Journal of Breeding 39(3): 365-372, 1989

A maize viviparous 1 gene increases seed dormancy and preharvest sprouting tolerance in transgenic wheat. Journal of Cereal Science 55(2): 166-173, 2012

Characterization of the rich haplotypes of Viviparous-1A in Chinese wheats and development of a novel sequence-tagged site marker for pre-harvest sprouting resistance. Molecular Breeding 33(1): 75-88, 2014

Pre-harvest sprouting tolerance of different winter triticale cultivars in the Baltic Sea area. Acta Agriculturae Scandinavica Section B Soil and Plant Science 58(1): 11-16, 2008

Resistance of winter wheat cultivars to pre-harvest sprouting. Sjemenarstvo 15(5): 261-268, 1998

Methods for assessment of pre-harvest sprouting in wheat cultivars. Pesquisa Agropecuária Brasileira 47(7): 928-933, 2012

Pre-harvest sprouting resistance and associated components in 12 wheat cultivars. Cereal Research Communications 4(2): 173-179, 1976