+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Isolation and characterization of proteinases from the sarcocarp of snake-gourd fruit

Isolation and characterization of proteinases from the sarcocarp of snake-gourd fruit

Journal of Biochemistry 99(2): 569-577

Seven proteinases were isolated from the fruit of snake-gourd, Trichosanthes cucumeroides Maxim. Their isozymes are all serine proteinases, and homologous in their respective molecular weights, amino acid compositions, and enzymatic properties. Their molecular weight was estimated to be about 50,000. Using casein as a substrate, the maximum activity was found in the alkaline pH region. The optimum temperature using casein was 70 degrees C at pH 7.3. The enzymes were strongly inhibited by diisopropyl fluorophosphate and not inhibited by inhibitors of sulfhydryl or metalloproteases. The reduced and S-carboxymethylated insulin B-chain was used as a substrate in an investigation of the specificity. The enzyme was found to have a wide specificity for this substrate but preferentially hydrolyzed the peptide bonds involving the carboxyl groups of charged amino acid such as S-cm-cysteine, glutamic acid, histidine, arginine, and lysine. Experimental evidence indicated that the snake-gourd proteinases are similar in their properties to cucumisin, which is isolated from the sarcocarp of melon fruit.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 016206558

Download citation: RISBibTeXText

PMID: 3516984

Related references

Isolation and characterization of proteinases from the sarcocarp of snake gourd trichosanthes cucumeroides fruit. Journal of Biochemistry 99(2): 569-578, 1986

Proteases from the sarcocarp of yellow snake gourd. Phytochemistry 29(6): 1879-1882, 1990

Isolation and characterization of a proteinase from the sarcocarp of melon fruit. Journal of Biochemistry 78(6): 1287-1296, 1975

Improvement and cultivation: bitter gourd, snake gourd, pointed gourd, and ivy gourd. Cucurbits 198, 1998

Fruit rot of snake gourd. Agr Res J Kerala 3(1): 106, 1965

Insecticidal control of the fruit fly, Dacus cucurbitae Coq., on snake gourd. Madras agric. J 49: 309-10, 1962

Evaluation of potential control measure for fruit fly, Bactrocera (Dacus) cucurbitae, in snake gourd. Bangladesh Journal of Entomology 2: 31-34, 1992

Fruit quality of snake gourd (Trichosanthes anguina L.) as influenced by nitrogen, ethephon and drip irrigation frequency. Vegetable Science 26(2): 152-156, 1999

Studies on floral biology of snake gourd (Trichosanthes anguina L.) and ash gourd (Benincasa hispida Thunb. Cogn.). Mysore Journal of Agricultural Sciences 14(1): 8-10, 1980

Further characterization of the saccharide specificity of snake gourd (Trichosanthes anguina) seed lectin. Current Science 75(6): 608-611, 1998

Effect of pre-sown electrical stimulus of seed on growth and yield of ridge gourd (Luffa acutangula Roxb.) and snake gourd (Trichosanthes anguina L.). Indian Journal of Plant Physiology 11(3): 291-294, 2006

Evaluation and characterization of the seed oils of Trichosanthes cucumerina (snake gourd) and Ricinodendron heudelotii (honey plum). Global Journal of Pure and Applied Sciences 9(2): 217-220, 2003

Purification and characterization of two proteinases from bushmaster lachesis muta snake venom. Toxicon 28(6): 621-622, 1990