+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Nitrate reductase activity of free-living and symbiotic uptake hydrogenase-positive and uptake hydrogenase-negative strains of Bradyrhizobium japonicum



Nitrate reductase activity of free-living and symbiotic uptake hydrogenase-positive and uptake hydrogenase-negative strains of Bradyrhizobium japonicum



Archives of microbiology51(2): 166-170



The nitrate reductase activity (NR) of selected uptake hydrogenase-positive (hup+) and uptake hydrogenase-negative (hup-) strains of Bradyrhizobium japonicum were examined both in free-living cells and in symbioses with Glycine max L. (Marr.) cv. Williams. Bacteria were cultured in a defined medium containing either 10 mM glutamate or nitrate as the sole nitrogen source. Nodules and bacteriods were isolated from plants that were only N2-dependent or grown in the presence of 2 mM KNO3. Rates of activity in nodules were determined by an in vivo assay, and those of cultured cells and bacteriods were assayed after permeabilization of the cells with alkyltrimethyl ammonium bromide. All seven strains examined expressed NR activity as free-living cells and as symbiotic forms, regardless of the hup genotype of the strain used for inoculation. Although the presence of nitrate increased nitrate reduction by cultures cells and nodules, no differences in NR activity were observed between bacteroids isolated from nodules of plants fed with nitrate or grown on N2-fixation exclusively. Cultured cells, nodules and bacteriods of strains with hup- genotype (USDA 138, L-236, 3. 15B3 and PJ17) had higher rates of NR activity than those with hup+ genotype (USDA 110, USDA 122 DES and CB1003). These results suggest that NR activity is reduced in the presence of a genetic determinant associated with the hup region of B. japonicum.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 016485871

Download citation: RISBibTeXText

DOI: 10.1007/bf00414433


Related references

Differences in nitrate reductase activity between uptake hydrogenase positive and uptake hydrogenase negative strains of bradyrhizobium japonicum. Bothe, H , F J De Bruijn And W E Newton (Ed ) Nitrogen Fixation: Hundred Years After; 7th International Congress, Cologne, West Germany, March 13-20, Xxi+878p Vch Publishers, Inc : New York, New York, Usa; Vch Verlagsgesellschaft Mbh: Weinheim, West Germany; Gustav Fischer Verlag: Stuttgart, West Germany Illus 583, 1988

Nitrate reductase activity of free living and uptake hydrogenase negative stains bradyrhizobium japonicum. Archives of Microbiology 151(2): 166-170, 1989

Nitrate reductase activity in nodules of pea inoculated with hydrogenase positive and hydrogenase negative strains of rhizobium leguminosarum. Physiologia Plantarum 69(2): 313-316, 1987

Difference in survival of uptake hydrogenase positive and negative strains of Bradyrhizobium japonicum in flooded and non-flooded soil. Soil Science and Plant Nutrition 38(2): 361-364, 1992

Effect of hydrogen uptake positive and negative strains of rhizobium along with different nitrate levels on hydrogenase nitrogenase and nitrate reductase activities of mungbean vigna radiata l. Journal of Research Punjab Agricultural University 27(3): 457-462, 1990

Identification of a potential transcriptional regulator of hydrogenase activity in free-living Bradyrhizobium japonicum strains. Molecular and general genetics: MGG 239(1-2): 235-240, 1993

Stimulation of hydrogenase synthesis in free-living Bradyrhizobium japonicum by addition of nitrate and ammonium. Soil Science and Plant Nutrition 36(2): 345-347, 1990

Denitrification by bacteroids of an uptake hydrogenase negative (Hup-) mutant and its isogenic Hup+ parental strain of Bradyrhizobium japonicum. FEMS Microbiology Letters 77(2+3): 157-162, 1991

Carbon nutrition and the regulation of uptake hydrogenase activity in free living and symbiotic anabaena cycadeae. New Phytologist 104(1): 115-120, 1986

Selection of symbiotically energy efficient strains of rhizobium japonicum by their ability to induce a hydrogen uptake hydrogenase in the free living state. Archives of Microbiology 128(3): 275-279, 1981

Lack of carbon substrate repression of uptake hydrogenase activity in Bradyrhizobium japonicum SR. Journal of Bacteriology 170(4): 1962-1964, 1988

Engineering the Rhizobium leguminosarum bv. viciae hydrogenase system for expression in free-living microaerobic cells and increased symbiotic hydrogenase activity. Applied and Environmental Microbiology 68(5): 2461-2467, 2002

The construction of chimeric plasmid carrying hup genes from Bradyrhizobium japonicum and expression of H-2-uptake hydrogenase activity in Enterobacter cloacae. Acta Phytophysiologica Sinica 22(3): 209-217, 1996

Effects of carbaryl and 2,4-D on growth, nitrogenase and uptake hydrogenase activity in agar culture and root nodules formed by Bradyrhizobium japonicum. Microbiological Research 149(4): 401-406, 1994

On the mechanism of hydrogen evolution by the uptake hydrogenase of bradyrhizobium japonicum. Journal of Molecular Catalysis 53(3): L1-L4, 1989