Occurrence and biosynthesis of glyoxysomal enzymes in ripening cucumber seeds

Frevert, J.; Köller, W.; Kindl, H.

Hoppe-Seyler's Zeitschrift für Physiologische Chemie 361(10): 1557-1565

1980


ISSN/ISBN: 0018-4888
PMID: 7450675
DOI: 10.1515/bchm2.1980.361.2.1557
Accession: 016532320

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Abstract
Glyoxysomal enzymes, being necessary during seed germination, are already synthesized at the stage of seed maturation. Two stages of embryogenesis of cucumber seeds (Cucumis sativus) were investigated. One was characterized by the presence of microbodies showing catalase and enoyl-CoA hydratase activities. Microbodies at a later stage contained, in addition, malate synthase and isocitrate lyase. The biosynthesis of three microbody components was followed in a pulse chase-labelling experiment which demonstrated that the biosynthesis of cytosolic species of malate synthase, isocitrate lyase and enoyl-CoA hydratase preceded the appearance of these proteins in microbodies.