+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

PCR-RFLP analysis of non-coding regions of cpDNA in Araucaria angustifolia (Bert.) O. Kuntze



PCR-RFLP analysis of non-coding regions of cpDNA in Araucaria angustifolia (Bert.) O. Kuntze



Genetics and Molecular Biology 30(2): 423-427



The Araucaria angustifolia (Bert.) O. Kuntze, also named the "parana pine" ('pinheiro-do-Paran' in Portuguese), is a native conifer species naturally occurring in the Brazilian Tropical Atlantic Forest which in Brazil is mostly limited to the southern Brazilian states of Parana, Santa Catarina and Rio Grande do Sul. Chloroplast DNA markers (cpDNA) are useful in populational genetic studies because of their low substitution rate and the uniparental transmission. The conservation of cpDNA genes between species has allowed the design of consensus chloroplast primers that have had a great impact on population genetics and phylogenetic studies. In this study we used the polymerase chain reaction technique combined with restriction enzyme fragment length polymorphism (PCR-RFLP) to characterize the genetic diversity of the chloroplast genome in nine natural A. angustifolia populations. Among the 141 trees surveyed we found 12 different cpDNA haplotypes and demonstrated that A. angustifolia has high levels of total diversity (h(T) = 0.612) and an average within-population diversity (h(S)) of 0.441, suggesting the presence of high within-population variation. The estimated genetic divergence could be helpful in designing breeding programs and species conservation strategies, although additional studies with a larger number of populations and trees is essential for a better understanding of gene flow and the inheritance of major Araucaria angustifolia traits.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 016596363

Download citation: RISBibTeXText

DOI: 10.1590/s1415-47572007000300020


Related references

Araucaria angustifolia (Bert.) O. Kuntze in the Brazilian forest. Iheringia Serie botanica: (30) 85-96, 1983

Dispersion del polen en Araucaria angustifolia (Bert.) O. Kuntze. Revista del Museo Argentino de Ciencias Naturales Nueva Serie 5(2): 135-138, 2003

Use of low temperature for the conservation of seeds of Araucaria angustifolia (Bert.) O. Kuntze. II Simposio sobre avances en la produccion de semillas forestales en America Latina: Memorias, Santo Domingo, Re ica Dominicana, 18-22 de octubre 1999: 215-218, 2000

Araucaria angustifolia (Bert) O. Kuntze. (Synonym A. brasiliana A. Rich). Tech. Note For. Dep. Kenya. 107, 5, 1967

Refrigerated storage of Araucaria angustifolia (Bert.) O. Kuntze seeds. Bosque 20(2): 117-124, 1999

Somatic embryogenesis in Parana pine (Araucaria angustifolia (Bert.) O. Kuntze). Brazilian Archives of Biology and Technology 45(1): 97-106, 2002

Reproductive phenology and seed production of Araucaria angustifolia (Bert.) O. Kuntze. 2004

Refrigerated storage of seeds of Araucaria angustifolia (Bert.) O. Kuntze over a period of 24 months. Seed Science and Technology 31(2): 411-421, 2003

Genetic variation in natural populations of Araucaria angustifolia (Bert.) O. Kuntze in Brazil. Genetic response of forest systems to changing environmental conditions: 93-103, 2001

Growth of Araucaria angustifolia (Bert.) O. Kuntze in th e province of Buenos Aires, Argentina. Revista forestal argentina: 18 (2) 41-47, 1974

Antioxidant and Antigenotoxic Activities of the Brazilian Pine Araucaria angustifolia (Bert.) O. Kuntze. Antioxidants 3(1): 24-37, 2014

Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. Kuntze. Brazilian Archives of Biology and Technology 47(6): 873-879, 2004

Natural regeneration under Araucaria angustifolia (Bert.) O. Kuntze forest in the Genetic Reserve of Cacador-SC. Revista Arvore 22(2): 143-153, 1998

Propriedades físicas da madeira de araucaria angustifolia bert o kuntze e sua relacao com os aneis de crescimento. 2013

Possible prehistoric anthropogenic effect on Araucaria angustifolia (Bert.) O. Kuntze expansion during the late Holocene. Revista Brasileira de Paleontologia 9(1): 109-116, 2006