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Phenylalanyl-tRNA and seryl-tRNA synthetases from baker's yeast. Substrate specificity with regard to ATP analogs and mechanism of the aminoacylation reaction

Freist, W.; von der Haar, F.; Sprinzl, M.; Cramer, F.

European Journal of Biochemistry 64(2): 389-393

1976

ISSN/ISBN: 0014-2956
PMID: 776617
DOI: 10.1111/j.1432-1033.1976.tb10313.x
Accession: 016645023
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Eighteen analogs of ATP have been tested in the aminoacylation reaction of phenylalanyl-tRNA and seryl-tRNA synthetases from baker's yeast. Four compounds are substrates for phenylalanyl-tRNA synthetase, five for seryl-tRNA synthetase, one compound is an inhibitor for both enzymes; their Km and Ki and V values have been determined. The substrate specificity shows that for the catalytic action of both enzymes with these substrates positions 6, 7, 8 and 9 of the purine moiety and positions 2' and 3' of the ribose moiety are important.

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Related References

Wolfgang FREIST; Friedrich VON DER HAAR; Mathias SPRINZL; Friedrich CRAMER 1976: Phenylalanyl-tRNA and Seryl-tRNA from Synthetases Baker's Yeast : Substrate Specificity with Regard to ATP Analogs and Mechanism of the Aminoacylation Reaction Febs Journal 64(2): 389-393
Freist, W.; von der Haar, F.; Faulhammer, H.; Cramer, F. 1976: Valyl-tRNA, isoleucyl-tRNA and tyrosyl-tRNA synthetase from baker's yeast. Substrate specificity with regard to ATP analogs and mechanism of the aminoacylation reaction European Journal of Biochemistry 66(3): 493-497
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