+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Polymerase chain reaction for detection of peanut mottle and peanut stripe viruses in Arachis hypogaea L. germplasm seedlots

Polymerase chain reaction for detection of peanut mottle and peanut stripe viruses in Arachis hypogaea L. germplasm seedlots

Plant Pathology Journal Faisalabad 6(1): 87-90

Results of peanut mottle and peanut stripe virus detection studies indicated that peanut seedlots were best handled by extracting nucleic acid from samples of seed slices pooled from rows and columns of seeds arranged in a grid. A portion of these RNA samples were then combined (plate pooled sample) and tested via RT-PCR. This approach could detect one infected seed in a 96-seed sample.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 016694389

Download citation: RISBibTeXText

Related references

Reaction of Arachis germplasm to peanut stripe, peanut mottle and tomato spotted wilt viruses. Peanut Science 20(2): 115-118, 1993

Sensitive Method for Testing Peanut Seed Lots for Peanut stripe and Peanut mottle viruses by Immunocapture-Reverse Transcription-Polymerase Chain Reaction. Plant Disease 84(5): 559-561, 2000

Final report on the safety assessment of Peanut (Arachis hypogaea) Oil, Hydrogenated Peanut Oil, Peanut Acid, Peanut Glycerides, and Peanut (Arachis hypogaea) Flour. International Journal of Toxicology 20(Suppl. 2): 65-77, 2001

A specific qualitative detection method for peanut (arachis hypogaea) in foods using polymerase chain reaction. Journal of food biochemistry 30(2): 215-233, 2006

Effect of peanut mottle virus on reaction of peanut arachis hypogaea cultivar tamnut 74 to cercospora arachidicola. Peanut Science 13(1): 31-33, 1986

Transmission of peanut mottle virus by three aphid vectors from peanut arachis hypogaea to peanut in the laboratory. Virginia Journal of Science 37(1): 16-22, 1986

Quantitative detection of transgenes in soybean [Glycine max (L.) Merrill] and peanut (Arachis hypogaea L.) by real-time polymerase chain reaction. Plant Cell Reports 20(5): 422-428, 2001

Production of peanut seed free of peanut stripe and peanut mottle viruses in Florida. Plant Disease 77(7): 747-749, 1993

Nonradioactive detection of peanut mottle and peanut stripe viruses with cRNA probes transcribed from cloned 3 regions of the viral genomes. Reddy, D V R , McDonald, D , Moss, J P Working together on groundnut virus diseases 47, 1994

Testing peanut arachis hypogaea seeds for peanut stripe virus. Peanut Science 13(1): 38-40, 1986

Ultrastructural cytology of peanut arachis hypogaea cultivar florigiant infected with peanut stripe virus. Phytopathology 75(11): 1311, 1985

Translation Initiation Factor eIF4E and eIFiso4E Are Both Required for Peanut stripe virus Infection in Peanut ( Arachis hypogaea L.). Frontiers in Microbiology 8: 338, 2017

Peanut stripe potyvirus resistance in peanut (Arachis hypogaea L.) plants carrying viral coat protein gene sequences. Transgenic Research 13(1): 59-67, 2004

Screening peanut arachis hypogaea germ plasm lines by enzyme linked immunosorbent assay elisa for seed transmission of peanut mottle virus. Plant Disease 68(9): 757-758, 1984

Effect of 2 strains of peanut mottle virus on fatty acids amino acids and protein of 6 peanut arachis hypogaea lines. Peanut Science 6(2): 88-92, 1979