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Polymerase chain reaction for detection of peanut mottle and peanut stripe viruses in Arachis hypogaea L. germplasm seedlots



Polymerase chain reaction for detection of peanut mottle and peanut stripe viruses in Arachis hypogaea L. germplasm seedlots



Plant Pathology Journal Faisalabad 6(1): 87-90



Results of peanut mottle and peanut stripe virus detection studies indicated that peanut seedlots were best handled by extracting nucleic acid from samples of seed slices pooled from rows and columns of seeds arranged in a grid. A portion of these RNA samples were then combined (plate pooled sample) and tested via RT-PCR. This approach could detect one infected seed in a 96-seed sample.

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Accession: 016694389

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