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Potide-G derived from potato (Solanum tuberosum L.) is active against potato virus YO (PVYO) infection



Potide-G derived from potato (Solanum tuberosum L.) is active against potato virus YO (PVYO) infection



Journal of Agricultural and Food Chemistry 54(22): 8437-8443



A PVYO virus-resistant potato (Solanum tuberosum L. cv. Golden Valley) was identified, and further, from its tubers, a small (5.57 kDa) antiviral peptide potide-G was isolated. Application of potide-G on virus susceptible potato (cv. Winter valley) expressed robust resistance to PVYO infection and showed no virus infected morphology. We found that PVYO infection spreads up completely within 3 days post inoculation (dpi) in susceptible cultivar. PVYO was more accumulated toward the basal leaves, when infection occurred longer. Combined results of morphology of PVYO infection, ELISA, RT-PCR, and real-time PCR showed the resistance to the PVYO infection depends on the expression of Ry gene. Indeed, the real-time PCR result showed that the Ry gene up-regulated to 3 times higher in PVYO infected cv. Golden valley. Golden crude protein was found to be active against PVYO infection in the in vivo test. In addition, application of potide-G in a virus susceptible potato potently reduced the viral infection actively with 50 times lower concentration than that of the Golden protein. Further identification of a host-specific resistant gene in a plant and the peptide derived from it offers new opportunities for the development of novel bio-pesticides against plant virus.

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Accession: 016715490

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PMID: 17061818

DOI: 10.1021/jf061794p



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