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Preparation of monospecific antiserum to lupin nodule glutamate dehydrogenase


, : Preparation of monospecific antiserum to lupin nodule glutamate dehydrogenase. Australian Journal of Biological Sciences 34(2): 161-169

Immunization of rabbit, using biochemically homogeneous glutamate dehydrogenase, proved to be unsuitable to produce monospecific antiserum. The presence of traces of contaminating immunogen (undetected by physiochemical methods) induced the production of other antibodies. Procedures for rigorously establishing monospecificity of antisera and a technique for preparation of monospecific antiserum, using immunologically impure antigens, are described.

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Accession: 016734375

PMID: 7283876

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