+ Translate
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Prevalence of Shiga Toxin-Producing Escherichia coli stx subscript 1(B, stx subscript 2(B, eaeA, and rfbE Genes and Survival of E. coli O157:H7 in Manure from Organic and Low-Input Conventional Dairy Farms

Prevalence of Shiga Toxin-Producing Escherichia coli stx subscript 1(B, stx subscript 2(B, eaeA, and rfbE Genes and Survival of E. coli O157:H7 in Manure from Organic and Low-Input Conventional Dairy Farms

Applied and environmental microbiology AEM1 73(7): 2180-2190

Manure samples were collected from 16 organic (ORG) and 9 low-input conventional (LIC) Dutch dairy farms during August and September 2004 to determine the prevalence of the STEC virulence genes stx subscript 1(B (encoding Shiga toxin 1), stx subscript 2(B (encoding Shiga toxin 2), and eaeA (encoding intimin), as well as the rfbE gene, which is specific for Escherichia coli O157. The rfbE gene was present at 52% of the farms. The prevalence of rfbE was higher at ORG farms (61%) than at LIC farms (36%), but this was not significant. Relatively more LIC farms were positive for all Shiga toxin-producing E. coli (STEC) virulence genes eaeA, stx subscript 1(B, and stx subscript 2(B, which form a potentially highly virulent combination. Species richness of Enterobacteriaceae, as determined by DGGE, was significantly lower in manure positive for rfbE. Survival of a green fluorescent protein-expressing E. coli O157:H7 strain was studied in the manure from all farms from which samples were obtained and was modeled by a biphasic decline model. The time needed to reach the detection limit was predominantly determined by the level of native coliforms and the pH (both negative relationships). Initial decline was faster for ORG manure but leveled off earlier, resulting in longer survival than in LIC manure. Although the nonlinear decline curve could theoretically be explained as the cumulative distribution of an underlying distribution of decline kinetics, it is proposed that the observed nonlinear biphasic pattern of the survival curve is the result of changing nutrient status of the manure over time (and thereby changing competition pressure), instead of the presence of subpopulations differing in the level of resistance.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 016742517

Download citation: RISBibTeXText

Related references

Prevalence of Shiga toxin-producing Escherichia coli stx1, stx2, eaeA, and rfbE genes and survival of E. coli O157:H7 in manure from organic and low-input conventional dairy farms. Applied and Environmental Microbiology 73(7): 2180-2190, 2007

Antimicrobial susceptibility of Shiga toxin-producing Escherichia coli isolated from organic dairy farms, conventional dairy farms, and county fairs in Minnesota. Foodborne Pathogens and Disease 4(2): 178-186, 2007

Prevalence of shiga toxin-encoding bacteria and shiga toxin-producing Escherichia coli isolates from dairy farms and county fairs. Veterinary Microbiology 118(3-4): 289-298, 2006

Prevalence of asymptomatic carriers of Shiga toxin-producing Escherichia coli (STEC) in dairy cattle farms in the governorate of Blida (Algeria). Bulletin of the Veterinary Institute in Pulawy 59(1): 23-28, 2015

A Four-Plex Real-Time PCR Assay, Based on rfbE, stx1, stx2, and eae Genes, for the Detection and Quantification of Shiga Toxin-Producing Escherichia coli O157 in Cattle Feces. Foodborne Pathogens and Disease 12(9): 787-794, 2015

Prevalence, serotypes and virulence genes of Shiga toxin-producing Escherichia coli isolated from ovine and caprine milk and other dairy products in Spain. International Journal of Food Microbiology 107(2): 212-217, 2006

Growth and survival of non-O157:H7 Shiga-toxin-producing Escherichia coli in cow manure. Journal of Applied Microbiology 102(1): 89-99, 2007

Shiga toxin-producing Escherichia coli (STEC) strains on French dairy farms. 24th World Buiatrics Congress, Nice, France, 15-19 October 2006: 453-459, 2006

Isolation of Two Seven-Membered Ring C subscript 58 Fullerene Derivatives: C subscript 58F subscript 17CF subscript 3 and C subscript 58F subscript 18. Science 8 309(5732): 278-281, 2005

Prevalence and characterization of Shiga toxin-producing Escherichia coli O157 and O26 in beef farms. Veterinary Microbiology 150(1-2): 140-145, 2011

Molecular Screening and Characterization of Shiga Toxin-producing Escherichia coli By Multiplex PCR Assays for stx(1), stx(2), eaeA, H7 in Raw Milk. Kafkas Universitesi Veteriner Fakultesi Dergisi 25(2): 271-275, 2019

Prevalence of shiga toxin-producing Escherichia coli in dairy cattle and their products. Journal of Dairy Science 88(2): 450-465, 2005

Dissemination and persistence of Shiga toxin-producing Escherichia coli (STEC) strains on French dairy farms. Veterinary Microbiology 117(2-4): 180-191, 2006

Diversity and relatedness of Shiga toxin-producing Escherichia coli and Campylobacter jejuni between farms in a dairy catchment. Epidemiology and Infection 144(7): 1406-1417, 2016

Prevalence and behavior of multidrug-resistant shiga toxin-producing Escherichia coli, enteropathogenic E. coli and enterotoxigenic E. coli on coriander. Food Microbiology 59: 97-103, 2016