Production of Fusarium solani f. sp. pisi cutinase in Fusarium venenatum A3/5
Sørensen, J.Dam.; Petersen, E.I.; Wiebe, M.G.
Biotechnology Letters 29(8): 1227-1232
ISSN/ISBN: 0141-5492 PMID: 17505784 DOI: 10.1007/s10529-007-9369-7
Fusarium venenatum A3/5 was transformed using the Aspergillus niger expression plasmid, pIGF, in which the coding sequence for the F. solani f. sp. pisi cutinase gene had been inserted in frame, with a KEX2 cleavage site, with the truncated A. niger glucoamylase gene under control of the A. niger glucoamylase promoter. The transformant produced up to 21 U cutinase 1(-1) in minimal medium containing glucose or starch as the primary carbon source. Glucoamylase (165 U1(-1) or 8 mg 1(-1)) was also produced. Both the transformant and the parent strain produced cutinase in medium containing cutin.