Section 17
Chapter 16,772

Production of Fusarium solani f. sp. pisi cutinase in Fusarium venenatum A3/5

Sørensen, J.Dam.; Petersen, E.I.; Wiebe, M.G.

Biotechnology Letters 29(8): 1227-1232


ISSN/ISBN: 0141-5492
PMID: 17505784
DOI: 10.1007/s10529-007-9369-7
Accession: 016771769

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Fusarium venenatum A3/5 was transformed using the Aspergillus niger expression plasmid, pIGF, in which the coding sequence for the F. solani f. sp. pisi cutinase gene had been inserted in frame, with a KEX2 cleavage site, with the truncated A. niger glucoamylase gene under control of the A. niger glucoamylase promoter. The transformant produced up to 21 U cutinase 1(-1) in minimal medium containing glucose or starch as the primary carbon source. Glucoamylase (165 U1(-1) or 8 mg 1(-1)) was also produced. Both the transformant and the parent strain produced cutinase in medium containing cutin.

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