EurekaMag
+ Translate
+ Most Popular
Sobre os generos Lopadusa Stal e Bothrocoris Mayr (Hemiptera, Pentatomidae, Pentatomini)
RFEF pilot plant for inactivation of Escherichia coli in apple juice
Calpionella alpina Lorenz als "Leitfossil"
Sooretama: The Atlantic Rain Forest of Brazil. Francis Dov Por
Novel approaches in myeloma therapy
Preliminary Notes on the Study of Mitochondria During the 1st Stages of the Destruction of Cadavers
A preliminary study of the biology of lichee stink bug, Tessaratoma papulosa Drury, and its control
The status of the Sabu Island python - Die Situation des Savu-Pythons
Randomised trial of intravenous immunoglobulin as prophylaxis against infection in plateau-phase multiple myeloma. the UK Group for Immunoglobulin Replacement therapy in Multiple Myeloma
Conflicts between children
Clinical presentations in neuropsychiatry
Method of suturing a perforated ulcer of the stomach and duodenum
Identification of mechanical asphyxiation in cases of attempted masking of the homicide
Effects of endogenous ascorbate on oxidation, oxygenation, and toxicokinetics of cell-free modified hemoglobin after exchange transfusion in rat and guinea pig
Is it warm? Is it real? Or just low spatial frequency?
Additions and corrections to the list of Korean Heteroptera
Factors contributing to the maintenance of oligotrophy in an alpine glacial moraine lake (Wallowa lake) in Northeast Oregon
Classification, paleoecology, and biostratigraphy of crinoids from the Stull Shale (Late Pennsylvanian) of Nebraska, Kansas, and Iowa
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Neue Formen der Bauchsammler aus Turkmenien (Hymenoptera, Apoidea)
Atypische Hühnerpest und Newcastle-Krankheit. Ein Identifizierungsversuch
Parasitellus fucorum in the debris of Apis mellifera - a risk of confusion with Tropilaelaps spp
Applying the Technology Acceptance Model to the introduction of healthcare information systems
Ein akarologischer Nachruf für den "Roland"
Mutation research on crop plants in Argentina

Purification and properties of two xylanases from Macrotermes mulleri (Termitidae, Macrotermitinae) and its symbiotic fungus Termitomyces sp


Purification and properties of two xylanases from Macrotermes mulleri (Termitidae, Macrotermitinae) and its symbiotic fungus Termitomyces sp



Insect Biochemistry 187: 709-715



Two xylanases had been purified, the first from termite Macrotermes muelleri workers (xylanase XT) and the second from its symbiotic fungus Termitomyces sp. (xylanase XF) through successive liquid chromatographic steps on hydroxyapatite, DEAE sepharose and Mono PTM. The enzyme preparations were homogeneous as judged by gel electrophoresis. A 400-fold increase in specific activity (from the crude extract) was obtained. The apparent molecular weight of both xylanases determined by SDS-PAGE was 28.5 kDa. Their optimum ws 5.2 and their Km value determined with CMC as substrate was 0.5 .times. 10-3%. The enzymatic specificity, the glycoprotein nature, the thermal stability of both enzymes and the effect of various cations on activity were examined. The comparison of kinetic data and physical properties suggested that xylanase XT and xylanase XF were identical.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 016816758

Download citation: RISBibTeXText

Related references

Purification and properties of cellulases from the termite Macrotermes mülleri (Termitidae, Macrotermitinae) and its symbiotic fungus Termitomyces sp. Comparative Biochemistry and Physiology. B. Comparative Biochemistry 91(3): 449-458, 1988

Purification and properties of two xylanases from macrotermes muelleri termitidae macrotermitinae and its symbiotic fungus termitomyces sp. Insect Biochemistry 18(7): 709-716, 1988

Purification and properties of cellulases from the termite macrotermes muelleri termitidae macrotermitinae and its symbiotic fungus termitomyces sp. Comparative Biochemistry and Physiology B 91(3): 449-458, 1988

Purification of an endocellulase from Macrotermes bellicosus Termitidae, Macrotermitinae and from its symbiotic fungus Termitomyces sp Purification dune endocellulase chez Macrotermes bellicosus Termitidae - Macrotermitinae et chez son champignon symbiotique Termitomyces sp. Actes des Colloques Insectes Sociaux 10: 93-99, 1996

Purification and properties of the xylanases from the termite Macrotermes bellicosus and its symbiotic fungus Termitomyces sp. Comparative Biochemistry and Physiology. Part B Biochemistry and Molecular Biology 112(4): 629-635, 1995

Research and comparative study of proteasic activities of neutral castes of termite Macrotermes subhyalinus (Termitidae, Macrotermitinae) and its symbiotic fungus Termitomyces sp. Tropicultura 22(3): 151-155, 2004

Etude comparative entre la -glucosidase presente dans le tube digestif du termite Macrotermes mulleri (Termitidae, Macrotermitinae) et la -glucosidase du champignon symbiotique Termitomyces sp. Actes des Colloques Insectes Sociaux 3: 109-118, 1986

Comparison between the osidases of the termite Macrotermes mulleri and those of its symbiotic fungus Termitomyces sp. Comptes Rendus de l' Academie des Sciences Series 3, Sciences de la Vie 306(3): 115-120, 1989

Synergistic activities of the enzymes involved in cellulose degradation, purified from Macrotermes mülleri and from its symbiotic fungus Termitomyces sp. Comparative Biochemistry and Physiology. B. Comparative Biochemistry 91(3): 459-465, 1988

Essai d'interpretation de la symbiose digestive chez Macrotermes mulleri (Termitidae, Macrotermitinae). Actes des Colloques Insectes Sociaux 4: 111-118, 1988

Comparisons of enzymatic activities of two allopatric populations of Macrotermes bellicosus Termitidae Macrotermitinae Comparaisons des activites enzymatiques de deux populations allopatriques de Macrotermes bellicosus Termitidae Macrotermitinae. Insect Science and its Application. July-September; 223: 193-198, 2002

Properties of two β-glucosidases purified from the termite Macrotermes muelleri and from its symbiotic fungus Termitomyces sp. Carbohydrate Research 233: 273-278, 1992

Properties of two beta -glucosidases purified from the termite Macrotermes muelleri and from its symbiotic fungus Termitomyces sp. Carbohydrate Research 233: 273-278, 1992

The inoculation of newly formed fungus comb with Termitomyces in Macrotermes colonies (Isoptera, Macrotermitinae). Insectes Sociaux 364: 328-338, 1989

A new pattern of lignin degradation in the fungus comb of Macrotermes carbonarius (Isoptera, Termitidae Macrotermitinae). Sociobiology 34(3): 591-596, 1999