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Purification, crystallization and preliminary X-ray diffraction analysis of water-soluble chlorophyll-binding protein from Chenopodium album



Purification, crystallization and preliminary X-ray diffraction analysis of water-soluble chlorophyll-binding protein from Chenopodium album



Acta Crystallographica. Section F Structural Biology and Crystallization Communications 63(Pt 9): 740-741



A water-soluble chlorophyll-binding protein (WSCP) with photoconvertibility from Chenopodium album was extracted, purified and crystallized in a darkroom. Green crystals suitable for data collection appeared in about 10 d. A native data set was collected to 2.0 A resolution at 100 K. The space group of the crystal was determined to be orthorhombic I222 or I2(1)2(1)2(1), with unit-cell parameters a = 48.13, b = 60.59, c = 107.21 A. Preliminary analysis of the X-ray data indicated that there is one molecule per asymmetric unit.

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Accession: 016818142

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PMID: 17768342

DOI: 10.1107/s1744309107035658


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