+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Regulation of NADP-malate dehydrogenase in C4 plants: relationship among enzyme activity, NADPH to NADP ratios, and thioredoxin redox states in intact maize mesophyll chloroplasts



Regulation of NADP-malate dehydrogenase in C4 plants: relationship among enzyme activity, NADPH to NADP ratios, and thioredoxin redox states in intact maize mesophyll chloroplasts



Archives of Biochemistry and Biophysics 249(1): 171-179



NADP-malate dehydrogenase activity, the ratio of NADPH to NADP, and thioredoxin redox state in Zea mays chloroplasts were determined after various treatments. Following transfer from dark to light, NADP-malate dehydrogenase was activated more than 20-fold within 10 min while the proportion of pyridine nucleotide as NADPH increased from about 25 to 90%, and the proportion of thioredoxin in the reduced form increased from 20 to more than 90%, in less than 1 min. After transfer back to the dark, NADPH levels dropped very rapidly to the initial values recorded before illumination, while enzyme activity and reduced thioredoxin levels decreased more slowly. Addition of oxaloacetate or 3-phosphoglycerate to illuminated chloroplasts results in a decrease of about 70% in the activity of NADP-malate dehydrogenase, a 30% decrease in the level of NADPH, and a 25% decrease in the reduced thioredoxin content. Adding dihydroxyacetone phosphate and pyruvate had no effect. These results are considered in relation to the hypothesis that NADP-malate dehydrogenase activity in chloroplasts may be determined by factors regulating the ratio of NADPH to NADP as well as those influencing the redox state of thioredoxin.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 016877828

Download citation: RISBibTeXText

PMID: 3740850

DOI: 10.1016/0003-9861(86)90572-2


Related references

Regulation of nadp malate dehydrogenase in 4 carbon plants relationship among enzyme activity nadph to nadp ratios and thioredoxin redox states in intact maize mesophyll chloroplasts. Archives of Biochemistry & Biophysics 249(1): 171-179, 1986

Regulation of NADP-malate dehydrogenase in C4 plants: effect of varying NADPH to NADP ratios and thioredoxin redox state on enzyme activity in reconstituted systems. Archives of Biochemistry and Biophysics 249(1): 164-170, 1986

Regulation of nadp malate dehydrogenase in 4 carbon plants effect of varying nadph to nadp ratios and thioredoxin redox state on enzyme activity in reconstituted systems. Archives of Biochemistry & Biophysics 249(1): 164-170, 1986

Modulation of NADP-Malate Dehydrogenase Activity in Maize Mesophyll Chloroplasts. Plant Physiology 71(3): 513-518, 1983

Control of the Quantum Efficiencies of Photosystems I and Ii, Electron Flow, and Enzyme Activation following Dark-to-Light Transitions in Pea Leaves: Relationship between Nadp/Nadph Ratios and Nadp-Malate Dehydrogenase Activation State. Plant Physiology 99(3): 979-986, 1992

Control of the quantum efficiencies of photosystems i and ii electron flow and enzyme activation following dark to light transitions in pea leaves relationship between nadp nadph ratios and nadp malate dehydrogenase activation state. Plant Physiology 99(3): 979-986, 1992

Regulation of NADP-malate dehydrogenase in C-4 plants: Activity and properties of maize thioredoxin m and the significance of non-active site thiol groups. Australian Journal of Plant Physiology 22(4): 577-584, 1995

Modulation of nadp malate dehydrogenase activity in maize zea mays cultivar kelvedon glory mesophyll chloroplasts. Plant Physiology 71(3): 513-518, 1983

Regulation of nadp malate dehydrogenase by the light actuated ferredoxin thioredoxin system of chloroplasts. Febs Letters 81(2): 253-258, 1977

Regulation of 4 carbon pathway photosynthesis regulation of activation and inactivation of nadp malate dehydrogenase by nadp and nadph. Archives of Biochemistry & Biophysics 227(2): 416-424, 1983

Regulation of C4 photosynthesis: regulation of activation and inactivation of NADP-malate dehydrogenase by NADP and NADPH. Archives of Biochemistry and Biophysics 227(2): 416-424, 1983

Adaptation of tobacco plants to elevated CO2: influence of leaf age on changes in physiology, redox states and NADP-malate dehydrogenase activity. Journal of Experimental Botany 50(334): 665-675, 1999

Cloning and expression of the 4 carbon pathway enzymes nadp malic enzyme and nadp malate dehydrogenase from maize. Plant Physiology 83(4 Suppl.): 129, 1987

Redox regulation of maize NADP-malic enzyme by thiol-disulfide interchange: Effect of reduced thioredoxin on activity. Biochimica et Biophysica Acta 1206(1): 10-16, 1994

Transgenic potato plants with altered levels of chloroplast NADP-malate dehydrogenase: Interactions between photosynthetic electron transport and malate metabolism in leaves and in isolated intact chloroplasts. Planta (Berlin) 207(1): 105-114, 1998