EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of hydrogen utilisation in Rhizobium japonicum by cyclic AMP


Biochimica et Biophysica Acta 584(3): 479-492
Regulation of hydrogen utilisation in Rhizobium japonicum by cyclic AMP
Utilisation (uptake) of hydrogen gas by whole cells of Rhizobium japonicum was found to be influenced by the carbon source(s) present in the growth medium, with activity being highest in a medium containing sugars. Tricarboxylic acid cycle intermediates, such as malate, significantly reduced H2 utilisation. No reduction in the hydrogenase activity is observed when the enzyme is assayed directly by the tritium exchange method, indicating that the decrease in hydrogen uptake activity is not due to repression of hydrogenase biosynthesis. Cyclic AMP was found to alleviate the inhibition of H2 uptake by malate, and this requires new protein synthesis. Addition of chloramphenicol or rifampicin simultaneously with cyclic AMP eliminated the stimulation of H2 uptake in the malate medium. These results show that in R. japonicum cyclic AMP plays a major role in the regulation of H2 metabolism.


Accession: 016878962

PMID: 222344

DOI: 10.1016/0304-4165(79)90121-1



Related references

Regulation of hydrogen utilization in rhizobium japonicum by cyclic amp. Biochimica et Biophysica Acta 584(3): 479-492, 1979

Rhizobium japonicum mutant strains altered in regulation of hydrogen oxidation by oxygen. Abstracts of the Annual Meeting of the American Society for Microbiology 82: ABSTRACT K102, 1982

The role of ammonia l glutamate and cyclic amp in the regulation of ammonia assimilation in rhizobium japonicum. Biochimica et Biophysica Acta 538(2): 244-248, 1978

The role of ammonia, L-glutamate, and cyclic adenosine 3',5'-monophosphate in the regulation of ammonia assimilation in Rhizobium japonicum. Biochimica et Biophysica Acta 538(2): 244-248, 1978

B 12 coenzyme dependent enz ribo nucleotide reductase in rhizobium species and the effects of cobalt deficiency on the activity of the enzyme rhizobium leguminosarum rhizobium trifolii rhizobium phaseoli rhizobium japonicum rhizobium meliloti lactobacillus. Journal of Bacteriology 97(3): 1460-1465, 1969

Autotrophic growth of hydrogen uptake positive strains of rhizobium japonicum in an atmosphere supplied with hydrogen gas. Proceedings of the National Academy of Sciences of the United States of America 76(4): 1788-1792, 1979

The use of the technique of van schreven for the taxonomy of rhizobium strains streptomyces lavendulae streptomyces scabies streptomyces griseolus rhizobium meliloti rhizobium trifolii rhizobium leguminosarum rhizobium phaseoli rhizobium lupini rhizobium japonicum. Acta Microbiologica Polonica 17(3): 263-267, 1968

Hydrogen stimulated carbon di oxide fixation and coordinate induction of hydrogenase and ribulose bi phosphate carboxylase in a hydrogen uptake positive strain of rhizobium japonicum. Archives of Microbiology 123(1): 1-8, 1979

Effect of cyclic gmp in rhizobium japonicum. Abstracts of the Annual Meeting of the American Society for Microbiology 82: ABSTRACT K76, 1982

Genetics of hydrogen oxidation in Rhizobium japonicum. Biotecnologia en las Americas II Aplicaciones en agricultura tropical (Session 4, paper 5): 3, 1985