+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Restitution of hemagglutinating activity to spikeless particles of HVJ (Sendai virus) by glycoprotein components of Newcastle disease virus

Restitution of hemagglutinating activity to spikeless particles of HVJ (Sendai virus) by glycoprotein components of Newcastle disease virus

Microbiology and Immunology 21(7): 379-389

Spikeless particles of HVJ (Sendai virus) lacking in hemagglutinating (HA) activity were obtained by enzymatic digestion of virions with trypsin followed by centrifugation through a sucrose gradient. When they were mixed with glycoprotein components of Newcastle disease virus (NDV) obtained by treatment of purified virions with deoxycholate (DOC), the mixture showed hemagglutination reaction, which was inhibited by anti-NDV serum, but not by anti-HVJ serum. Sedimentation profile of the HA active agents was then examined by centrifugation of the mixture of spikeless particles of HVJ (labeled with 3H-uridine) and glycoproteins of NDV (labeled with 14C-amino acid mixture). The results showed that the peak of HA activity had both of the radioactivities, and that the sedimentation rate of the HA was faster than that of spikeless HVJ but slower than that of intact HVJ. Electron micrographs of such HA active structures showed that they were morphologically closely similar to intact virion of HVJ, although they had neither hemolytic activity nor infectivity. The mixture of spikeless HVJ and glycoproteins of HVJ or NDV which were removed from virions by proteolytic enzymes, on the other hand, did not show any detectable hemagglutinating activity.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 016928004

Download citation: RISBibTeXText

PMID: 198639

DOI: 10.1111/j.1348-0421.1977.tb00302.x

Related references

Gangliosides as paramyxovirus receptor. Structural requirement of sialo-oligosaccharides in receptors for hemagglutinating virus of Japan (Sendai virus) and Newcastle disease virus. Journal of Biochemistry 97(4): 1189-1199, 1985

Role of intracellular proteinases and glycosidases in the degradation of the 'large glycoprotein' responsible for the neuraminidase and hemagglutinating activity of the Newcastle disease virus. Voprosy Meditsinskoi Khimii 22(2): 266-269, 1976

Restitution of infectivity to spikeless vesicular stomatitis virus by solubilized viral components. Journal of Virology 16(1): 75-84, 1975

Identification of a glycoprotein inhibitor of human serum, active towards the hemagglutinating activity of Sendai virus. Bollettino Dell'istituto Sieroterapico Milanese 57(6): 700-706, 1979

Immunological studies on the antigenic determinants of sendai virus hn glycoprotein involved in hemagglutinating activity. Fems Microbiology Letters: 129-134, 1987

Revertant analysis of a temperature-sensitive mutant of Newcastle disease virus with defective glycoproteins: implication of the fusion glycoprotein in cell killing and isolation of a neuraminidase-deficient hemagglutinating virus. Journal of Virology 42(2): 659-668, 1982

Interferon synthesis in x irradiated animals iii the high radio sensitivity of myxovirus induced circulating interferon production mouse newcastle disease virus influenza a virus sendai virus mumps virus sindbis virus semliki forest virus vesicular stomatitis virus encephalo myo carditis virus vaccinia virus. Proceedings of the Society for Experimental Biology & Medicine 131(1): 36-41, 1969

Isolation and characteristics of the hemagglutinating components of Newcastle disease virus. ZEIT NATURFORSCH 16b(9): 625, 1961

Infective and noninfective hemagglutinating particles of Newcastle disease virus: biological and chemical characterization. Journal of Virology 16(5): 1191-1199, 1975

Studies on factors effecting the production of non infectious hemagglutinating particles of newcastle disease virus. Zhonghua Weishengwuxue He Mianyixue Zazhi 5(1): 55-58, 1985

Isolation and characterization of hemagglutinating noninfectious particles produced during multiplication of Newcastle disease virus (NDV). Virology 16: 207-209, 1962

Studies on the interaction of hemagglutinating virus of japan sendai virus with liposomal membranes hemagglutinating virus of japan induced permeability increase of liposomes containing glycophorin. Virology 116(2): 419-427, 1982

Studies on the interaction of the large and small hemagglutinating components of Newcastle disease virus with red cells. Journal of Immunology 72(4): 329-339, 1954

An intra cellular interaction between a temperature sensitive mutant and the original wild type hemagglutinating virus of japan sendai virus is responsible for the establishment and maintenance of hemagglutinating virus of japan persistent infection. Journal of General Virology 55(2): 459-468, 1981

Long-term and memory immune responses in mice against Newcastle disease virus-like particles containing respiratory syncytial virus glycoprotein ectodomains. Journal of Virology 86(21): 11654-11662, 2012