+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Revisited ITS2 phylogeny of Anopheles (Anopheles) Hyrcanus group mosquitoes: reexamination of unidentified and misidentified ITS2 sequences



Revisited ITS2 phylogeny of Anopheles (Anopheles) Hyrcanus group mosquitoes: reexamination of unidentified and misidentified ITS2 sequences



Parasitology Research 101(4): 885-894



The Anopheles (Anopheles) Hyrcanus group of mosquitoes is very important for human health because some are malarial vector mosquitoes. Despite their pathological importance, some unidentified internal transcribed spacer 2 (ITS2) sequences have been reported from members of this group, and their phylogenetic relationships have been rarely understood. In the present study, 84 ITS2 sequences for the Hyrcanus group members were retrieved from GenBank. The detailed sequence comparison unambiguously revealed that the unknown1 sequences of Li et al. (Zootaxa 939:1-8, 2005), YM-2004 (or sp.2) of Ma and Xu [J Med Entomol 42(4):610-619, 2005], Anopheles sp. of Ree et al. (2005), and Anopheles lesteri reported by Gao et al. [J Med Entomol 41(1):5-11, 2004] are identical to Anopheles belenrae [Rueda (Zootaxa 941:1-26, 2005)] and that YM-2003 (or sp.1) of Ma and Xu [J Med Entomol 42(4):610-619, 2005] is closely related to A. lesteri. In addition, some candidate species that may be synonymized are suggested in addition to the possibility that A. lesteri may be divided into at least three types: A, B, and C. The neighbor joining, maximum parsimony, and maximum likelihood trees show that the 23 examined Hyrcanus group members could be divided into four subgroups. The phylogenetic relationships among them are generally well resolved with high bootstrapping values (60-100%), and they are consistently supported by all three trees without any conflicts. These results may strongly suggest that the morphology-based groupings of the Hyrcanus group members should be seriously reconsidered. Further study must be conducted to address the following issues: the three or more multitypified A. lesteri; the unidentified YM-2003 closely related to A. lesteri; and the synonymies of Anopheles peditaeniatus/Anopheles nigerrimus, Anopheles kunmingensis/Anopheles liangshanensis, Anopheles pullus/Anopheles junlianensis, and Anopheles engarensis/Anopheles kleini.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 016943370

Download citation: RISBibTeXText

PMID: 17514381

DOI: 10.1007/s00436-007-0553-4


Related references

Molecular phylogeny of Anopheles hyrcanus group members based on ITS2 rDNA. Parasites and Vectors 10(1): 417, 2017

Analysis of the sequences of the ribosomal DNA ITS2 region for differentiating anopheline mosquitoes within hyrcanus group. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 22(5): 277-279, 2004

Analysis of the sequences of the ribosomal DNA ITS2 region for differenciating anopheline mosquitoes within hyrcanus group. Chinese Journal of Parasitology and Parasitic Diseases. October 30; 225: 277-279, 2004

DNA sequence analysis of the ribosomal DNA ITS2 region for the Anopheles punctulatus group of mosquitoes. Insect molecular biology 8(3): 381-390, 1999

Comparison of rDNA-ITS2 sequences and morphological characters of Anopheles kunmingensis and Anopheles langshanensis in China, with discussion on taxonomic status. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 18(2): 65-68, 2000

Comparison of rDNA-ITS2 sequences and morphological characters of Anopheles kunmingensis and Anopheles langshanensis in China, with discussion on taxonomic status. Chinese Journal of Parasitology and Parasitic Diseases 18(2): 65-68, 2000

The ITS2 ribosomal DNA of Anopheles beklemishevi and further remarks on the phylogenetic relationships within the Anopheles maculipennis group of species (Diptera: Culicidae). Parasitology Research 97(2): 118-128, 2005

Molecular identification of the members of the Anopheles annularis group of mosquitoes (Diptera : Culicidae) using ribosomal DNA ITS2 and domain-3. 2007

First Molecular Identification and Phylogeny of Moroccan Anopheles sergentii (Diptera: Culicidae) Based on Second Internal Transcribed Spencer (ITS2) and Cytochrome c Oxidase I (COI) Sequences. Vector Borne and Zoonotic Diseases 18(9): 479-484, 2018

Sequence analysis of ribosomal dna ITS2 region for Anopheles anthropophagus and Anopheles sinebsis. Zhongguo Xuexichongbing Fangzhi Zazhi 15(6): 410-414, 2003

Comparison of Anopheles darlingi populations based on ITS2 DNA sequences. Memorias do Instituto Oswaldo Cruz 93(Suppl. 2): 328, 1998

Laboratory studies on growth and developmental behavior of anopheles annularis anopheles pallidus and anopheles philippinensis anopheles hyrcanus anopheles subpictus algae. Bulletin of the Indian Society for Malaria & Other Communicable Diseases 4(1): 13-22, 1967

Characterization of ITS2 rDNA of Anopheles philippinensis and Anopheles nivipes (Diptera: Culicidae) from north-east India. Southeast Asian Journal of Tropical Medicine and Public Health 37(6): 1134-1138, 2006

Studies on the different types of Anopheles hyrcanus slnensls within the Anopheles hyrcanus group. II. Comparative morphological study of pupa. Acta Entomol Sinlca 14(2): 156-162, 1965

Studies on the different types of Anopheles (A.) Hyrcanus sinensis within the Anopheles (A.) hyrcanus group. 2. Comparative morphological study of pupa. Science Abstracts of China Biological Sciences 4(No. 1: 18, 1966