EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Role of the Vps34p-interacting protein Ade5,7p in hyphal growth and virulence of Candida albicans


Role of the Vps34p-interacting protein Ade5,7p in hyphal growth and virulence of Candida albicans



Microbiology 153(Part 7): 2351-2362



ISSN/ISBN: 1350-0872

PMID: 17600079

DOI: 10.1099/mic.0.2006/004028-0

The phosphatidylinositol (PtdIns) 3-kinase Vps34p of the human pathogenic yeast Candida albicans participates in virulence and in protein transport. In order to dissect these two functions, a search for proteins interacting with C. albicans Vps34p was performed using a yeast two-hybrid system. This study demonstrates the physical interaction between Vps34p and Ade5,7p, which is the bifunctional enzyme of the de novo purine nucleotide biosynthetic pathway. The interaction initially observed in a yeast two-hybrid system was confirmed in vitro with recombinant proteins. Given the complex formation between Ade5,7p and the virulence-regulating Vps34p, it was of interest to characterize the function of Ade5,7p in C. albicans. To this end, ade5,7 null mutants were generated. The resulting mutants were adenine deficient, and sensitive to the presence of metal ions. In addition, the ade5,7 null mutants were avirulent in a mouse model of systemic candidiasis, and showed reduced hyphal growth in an agar matrix under embedded conditions. In summary, Ade5,7p interacts with the multifunctional virulence regulator PtdIns 3-kinase Vps34p, and ade5,7 and vps34 null mutant strains show similar phenotypes regarding sensitivity to metal ions, hyphal growth and virulence.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 016957654

Download citation: RISBibTeXText

Related references

Phosphatidate phosphatase Pah1 has a role in the hyphal growth and virulence of Candida albicans. Fungal Genetics and Biology: Fg and B 124: 47-58, 2019

Role of transcription factor CaNdt80p in cell separation, hyphal growth, and virulence in Candida albicans. Eukaryotic Cell 9(4): 634-644, 2010

Derepressed hyphal growth and reduced virulence in a VH1 family-related protein phosphatase mutant of the human pathogen Candida albicans. Molecular Biology of the Cell 8(12): 2539-2551, 1997

Candida albicans hyphal formation and virulence: is there a clearly defined role?. Trends in Microbiology 6(3): 92-94, 1998

Gymnemic acids inhibit hyphal growth and virulence in Candida albicans. Plos one 8(9): E74189, 2013

Deficiency of D-erythroascorbic acid attenuates hyphal growth and virulence of Candida albicans. Infection and Immunity 69(6): 3939-3946, 2001

Asc1, a WD-repeat protein, is required for hyphal development and virulence in Candida albicans. Acta Biochimica et Biophysica Sinica 42(11): 793-800, 2010

Niche-specific requirement for hyphal wall protein 1 in virulence of Candida albicans. Plos one 8(11): E80842, 2013

Virulence and hyphal formation of Candida albicans require the Ste20p-like protein kinase CaCla4p. Current Biology: Cb 7(8): 539-546, 1997

The formin family protein CaBni1p has a role in cell polarity control during both yeast and hyphal growth in Candida albicans. Journal of Cell Science 118(Part 12): 2637-2648, 2005

Signaling through adenylyl cyclase is essential for hyphal growth and virulence in the pathogenic fungus Candida albicans. Molecular Biology of the Cell 12(11): 3631-3643, 2001

Crk1, a novel Cdc2-related protein kinase, is required for hyphal development and virulence in Candida albicans. Molecular and Cellular Biology 20(23): 8696-8708, 2000

A hyphal-specific chitin synthase gene (CHS2) is not essential for growth, dimorphism, or virulence of Candida albicans. Proceedings of the National Academy of Sciences of the United States of America 91(13): 6216-6220, 1994

Antiserum to a surface protein on the hyphal form of candida albicans binds to the protein product of a 15kb candida albicans complementary dna. Abstracts of the General Meeting of the American Society for Microbiology 91: 91, 1991

Candida albicans glutathione reductase downregulates Efg1-mediated cyclic AMP/protein kinase A pathway and leads to defective hyphal growth and virulence upon decreased cellular methylglyoxal content accompanied by activating alcohol dehydrogenase and glycolytic enzymes. Biochimica et Biophysica Acta. General Subjects 1861(4): 772-788, 2017