+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Screening and identification of a targeting peptide to hepatocarcinoma from a phage display peptide library



Screening and identification of a targeting peptide to hepatocarcinoma from a phage display peptide library



Molecular Medicine 13(5-6): 246-254



Ligands specific to cell surface receptors have been heavily investigated in cancer research. Phage display technology is a powerful tool in this field and may impact clinical issues including functional diagnosis and targeted drug delivery. In this study, a hepatocellular carcinoma cell line (HepG2) and a normal hepatocyte line (L-02) were used to carry out subtractive screening in vitro with a phage display-7 peptide library. After four rounds of panning, there was an obvious enrichment for the phages specifically binding to the HepG2 cells, and the output/input ratio of phages increased about 976-fold (from 0.3x 10(-7) to 292.8x 10(-7)). A group of peptides capable of binding specifically to the hepatoma cells were obtained, and the affinity of these peptides to the targeting cells and tissues was studied. Through a cell-based ELISA, immunocytochemical staining, immunohistochemical staining, and immunofluorescence, the S1 phage and synthetic peptide HCBP1 (sequence FQHPSFI) were shown to bind to the tumor cell surfaces of two hepatoma cell lines and biopsy specimens, but not to normal hepatocytes, other different cancer cells, or nontumor liver tissues. In conclusion, the peptide HCBP1 may be a potential candidate for targeted drug delivery in therapy of hepatoma cancer.

(PDF emailed within 1 workday: $29.90)

Accession: 016987778

Download citation: RISBibTeXText

PMID: 17622312


Related references

Screening and Identification of a Targeting Peptide to Hepatocarcinoma from a Phage Display Peptide Library. Molecular Medicine 13(5-6): 246-254, 2007

Identification of a peptide specifically targeting ovarian cancer by the screening of a phage display peptide library. Oncology Letters 11(6): 4022-4026, 2016

In vitro panning of a targeting peptide to hepatocarcinoma from a phage display peptide library. Biochemical and Biophysical Research Communications 342(3): 956-962, 2006

Screening and identification of a renal carcinoma specific peptide from a phage display peptide library. Journal of Experimental and Clinical Cancer Research 30: 105, 2012

Screening and identification of a peptide specifically targeted to NCI-H1299 from a phage display peptide library. Cancer Letters 281(1): 64-70, 2009

Identification of a novel skin penetration enhancement peptide by phage display peptide library screening. Molecular Pharmaceutics 9(5): 1320-1330, 2012

Screening and identification of a peptide specifically targeted to NCI-H1299 cells from a phage display peptide library. Molecular Medicine Reports 2(6): 1005-1010, 2009

Screening and identification of a specific peptide binding to hepatocellular carcinoma cells from a phage display peptide library. Journal of Peptide Science 20(3): 196-202, 2014

Screening and identification of polypeptides specifically binding to human B type natriuretic peptide from 12TM phage display peptide library. Nan Fang Yi Ke Da Xue Xue Bao 29(9): 1837-1839, 2011

Identification of calreticulin as a ligand of GABARAP by phage display screening of a peptide library. Febs Journal 274(21): 5543-5555, 2007

Screening and identification of CD13-binding peptides with phage display peptide library. Nan Fang Yi Ke Da Xue Xue Bao 30(4): 827-830, 2011

Screening of peptide inhibitors of acetylcholinesterase from 12-mer random phage display peptide library. Nan Fang Yi Ke Da Xue Xue Bao 26(7): 1053-1054, 2006

Screening of TACE peptide inhibitors from phage display peptide library. Journal of Huazhong University of Science and Technology. Medical Sciences 25(5): 473-476, 2006

Screening of antagonist peptide of BLyS from C7C phage display peptide library. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 20(4): 398-401, 2004

Screening of a specific peptide binding to VPAC1 receptor from a phage display peptide library. Plos One 8(1): E54264, 2013