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Sheath-flow cuvette for high-sensitivity laser-induced fluorescence detection in capillary electrophoresis

Sheath-flow cuvette for high-sensitivity laser-induced fluorescence detection in capillary electrophoresis

Applied Spectroscopy 61(7): 777-779

The sheath-How cuvette is a key component in a high-sensitivity post-column laser-induced fluorescence detector for capillary electrophoresis. Most designs are based on commercial cuvettes originally manufactured for use in a flow cytometer. In these devices, a quartz flow chamber is held in a stainless-steel fixture that is difficult to machine and subjects the cuvette to a torque when sealed, which frequently leads to damage of the flow chamber. In this report we present a design for a cuvette that may easily be constructed. This design uses compression to hold and seal the quartz flow chamber without applying torque. The system produces detection limits (3 sigma) of 115 yoctomoles (70 copies) for FQ-labeled carbonic anhydrase.

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Accession: 017025932

Download citation: RISBibTeXText

PMID: 17697473

DOI: 10.1366/000370207781393208

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