Studies on cellulase from Trichoderma reesei and its effect on pretreated cellulosic materials

Nagieb, Z.; Ghazi, I.; Kassim, E.

Journal of Applied Polymer Science 30(12): 4653-4658

1985


ISSN/ISBN: 0021-8995
DOI: 10.1002/app.1985.070301212
Accession: 017158632

Download citation:  
Text
  |  
BibTeX
  |  
RIS

Article/Abstract emailed within 0-6 h
Payments are secure & encrypted
Powered by Stripe
Powered by PayPal

Abstract
The stability of locally produced cellulase from T. reesei was studied. The enzyme solution was stable at temperatures up to 45°C over which a gradual inactivation occured until 55–60°C. A rapid loss of enzymatic activity was observed over 60°C and complete inactivation occurred at 70°C after only 1 h. The maximum enzymatic saccharification was obtained at 50°C and pH 6.5. The pretreatment of cellulosic wastes, bagasse, and sawdust from soft and hard wood was carried out in different solvents, chloroform, dioxan, dichloroethane, ethylacetate, and ethanol, for 8 h. These treatments increased the susceptibility of the cellulosic wastes to enzymatic saccharification. The bagasse was highly affected, followed by the sawdust from soft and hard wood, respectively.

Studies on cellulase from Trichoderma reesei and its effect on pretreated cellulosic materials