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The effect of oxygen on 14CO2 fixation in mesophyll cells isolated from Digitaria sanguinalis (L.) Scop. leaves



The effect of oxygen on 14CO2 fixation in mesophyll cells isolated from Digitaria sanguinalis (L.) Scop. leaves



Biochemical and Biophysical Research Communications 55(3): 850-856



Mesophyll cells were isolated from fully-expanded leaves of Digitaria sanguinalis (L.) Scop. by a combined maceration-filtration technique. In the presence of pyruvate, photosynthetic 14CO2 uptake in the isolated cells was not inhibited by atomospheric levels of oxygen. In contrast, superatmospheric levels of oxygen substantially inhibited the light-dependent fixation of 14CO2. These oxygen effects are similar to those observed with intact C4 leaves and suggest that the lack of inhibition of C4 photosynthesis by atmospheric levels of oxygen results from the relative oxygen-insensitivity of the phosphopyruvate carboxylase-CO2 pump in the mesophyll.

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Accession: 017359585

Download citation: RISBibTeXText

PMID: 4761087

DOI: 10.1016/0006-291x(73)91222-9


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