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The establishment and optimization of a highly efficient system of shoot regeneration from hypocotyl explants of Phyllanthus emblica L

The establishment and optimization of a highly efficient system of shoot regeneration from hypocotyl explants of Phyllanthus emblica L

Acta Horticulturae Sinica 34(3): 609-614

An efficient plant regeneration protocol for Phyllanthus emblica was achieved via organogenesis from callus tissues formed on hypocotyl explants in vitro. In this paper, several factors affecting in vitro regeneration of P. emblica were examined. The optimum medium for shoot regeneration was 1/2 MS medium containing 0.1 micro mol NAA, 5 micro mol benzyladenine, 40 g sucrose and 1.0 mg AgNO<sub>3</sub>/litre. The optimum explants were the upper hypocotyl segments of 28-day-old seedlings.

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