+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The multifunctional protein p54nrb/PSF recruits the exonuclease XRN2 to facilitate pre-mRNA 3' processing and transcription termination



The multifunctional protein p54nrb/PSF recruits the exonuclease XRN2 to facilitate pre-mRNA 3' processing and transcription termination



Genes and Development 21(14): 1779-1789



Termination of RNA polymerase II transcription frequently requires a poly(A) signal and cleavage/polyadenylation factors. Recent work has shown that degradation of the downstream cleaved RNA by the exonuclease XRN2 promotes termination, but how XRN2 functions with 3'-processing factors to elicit termination remains unclear. Here we show that XRN2 physically associates with 3'-processing factors and accumulates at the 3' end of a transcribed gene. In vitro 3'-processing assays show that XRN2 is necessary to degrade the downstream RNA, but is not required for 3' cleavage. Significantly, degradation of the 3'-cleaved RNA was stimulated when coupled to cleavage. Unexpectedly, while investigating how XRN2 is recruited to the 3'-processing machinery, we found that XRN2 associates with p54nrb/NonO(p54)-protein-associated splicing factor (PSF), multifunctional proteins involved in several nuclear processes. Strikingly, p54 is also required for degradation of the 3'-cleaved RNA in vitro. p54 is present along the length of genes, and small interfering RNA (siRNA)-mediated knockdown leads to defects in XRN2 recruitment and termination. Together, our data indicate that p54nrb/PSF functions in recruitment of XRN2 to facilitate pre-mRNA 3' processing and transcription termination.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 017429775

Download citation: RISBibTeXText

PMID: 17639083

DOI: 10.1101/gad.1565207


Related references

mRNA decapping factors and the exonuclease Xrn2 function in widespread premature termination of RNA polymerase II transcription. Molecular Cell 46(3): 311-324, 2012

Heterogeneous nuclear ribonucleoprotein (HnRNP) K genome-wide binding survey reveals its role in regulating 3'-end RNA processing and transcription termination at the early growth response 1 (EGR1) gene through XRN2 exonuclease. Journal of Biological Chemistry 288(34): 24788-24798, 2013

Human 5- #38;#62;3 exonuclease Xrn2 promotes transcription termination at co-transcriptional cleavage sites. Nature 432: 2-5, 2004

Human 5' fwdarw 3' exonuclease Xrn2 promotes transcription termination at co-transcriptional cleavage sites. Nature (London) 432(7016): 522-525, 2004

Effects of Transcription Elongation Rate and Xrn2 Exonuclease Activity on RNA Polymerase II Termination Suggest Widespread Kinetic Competition. Molecular Cell 60(2): 256-267, 2016

Paraspeckle protein p54nrb links Sox9-mediated transcription with RNA processing during chondrogenesis in mice. Journal of Clinical Investigation 118(9): 3098-3108, 2008

The essential N terminus of the Pta1 scaffold protein is required for snoRNA transcription termination and Ssu72 function but is dispensable for pre-mRNA 3'-end processing. Molecular and Cellular Biology 29(8): 2296-2307, 2009

A Tetrahymena Piwi bound to mature tRNA 3' fragments activates the exonuclease Xrn2 for RNA processing in the nucleus. Molecular Cell 48(4): 509-520, 2013

XRN2 Links Transcription Termination to DNA Damage and Replication Stress. Plos Genetics 12(7): E1006107, 2017

Swi6/HP1 recruits a JmjC domain protein to facilitate transcription of heterochromatic repeats. Molecular Cell 22(5): 681-692, 2006

Rat1 and Xrn2: The Diverse Functions of the Nuclear Rat1/Xrn2 Exonuclease. Enzymes 31: 131-163, 2012

Microprocessor, Setx, Xrn2, and Rrp6 co-operate to induce premature termination of transcription by RNAPII. Cell 150(6): 1147-1157, 2012

Connections between mRNA 3' end processing and transcription termination. Current Opinion in Cell Biology 17(3): 257-261, 2005

Exosome Cofactors Connect Transcription Termination to RNA Processing by Guiding Terminated Transcripts to the Appropriate Exonuclease within the Nuclear Exosome. Journal of Biological Chemistry 291(25): 13229-13242, 2016

P-TEFb regulation of transcription termination factor Xrn2 revealed by a chemical genetic screen for Cdk9 substrates. Genes and Development 30(1): 117-131, 2016