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Type I interferon prolongs cell cycle progression via p21WAF1/CIP1 induction in human colon cancer cells



Type I interferon prolongs cell cycle progression via p21WAF1/CIP1 induction in human colon cancer cells



International Journal of Oncology 31(3): 613-620



Type I interferon (IFN) was originally identified as an immunomodulatory cytokine because of its antiviral activity. Further characterization of its biological effects revealed a prominent role in the direct control of cell growth and potent immunomodulatory and antiangiogenic actions. IFN-alpha and IFN-SS had both been classified as type I IFN, but differences in their antitumor activities were reported. We confirmed the difference in the antiproliferative activities of IFN-alpha 2b and IFN-SS toward HT29 and SW480 cells. IFN treatment was observed to prolong cell cycle progression; in particular, the accumulation of S-phase population was one of the most characteristic changes. The prolongation of S-phase progression and transition into G2/M-phase was suggested to be a crucial action of type I IFN on colon cancer. Additionally, IFN activated the p21 promoter gene and induced p21(WAFl/CIP1) expression. Furthermore, the cell cycle prolongation effect of IFN was suppressed when p21 expression was downregulated. Therefore, we confirmed that p21(WAFl/CIP1) was a crucial target molecule for the effects of IFN on the cell cycle. Additionally, the ability of p21 induction differed between IFN-alpha 2b and IFN-SS and correlated with their inhibitory activities toward cell growth. We conclude that type I IFN prolongs cell cycle progression by p21(WAFl/CIP1) induction in human colon cancer cells.

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Accession: 017577378

Download citation: RISBibTeXText

PMID: 17671689

DOI: 10.3892/ijo.31.3.613


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