+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

1H NMR spectroscopy of Paracoccus denitrificans cytochrome c-550

1H NMR spectroscopy of Paracoccus denitrificans cytochrome c-550

Biochemistry 23(15): 3526-3533

The 1H NMR spectra of ferri- and ferro-cytochrome c-550 from Paracoccus denitrificans (ATCC 13543) have been investigated at 300 MHz. The ferri-cytochrome c-550 shows hyperfine-shifted heme methyl resonances at 29.90, 29.10, 16.70, and 12.95 ppm and a ligand methionyl methyl resonance at -15.80 ppm (pH 8 and 23 degrees C). Four pH-linked structural transitions were detected in spectra taken as a function of pH. The transitions have been interpreted as loss of the histidine heme ligand (pK less than or equal to 3), ionization of a buried heme propionate (pK = 6.3 +/- 0.2), displacement of the methionine heme ligand by a lysyl amino group (pK congruent to 10.5), and loss of the lysyl ligand (pK greater than or equal to 11.3). The temperature behavior of hyperfine-shifted resonances was determined. Two heme methyl resonances (at 16.70 and 12.95 ppm) showed downfield hyperfine shifts with increasing temperature. The cyanoferricytochrome had methyl resonances at 23.3, 20.1, and 19.4 ppm. NMR spectroscopy did not detect the formation of a complex with azide. The second-order rate constant for electron transfer between ferric and ferrous forms was determined to be 1.6 X 10(4) M-1 s-1. Heme proton resonances were assigned in both oxidation states by cross-saturation and nuclear Overhauser enhancement experiments. Spin-coupling patterns in the aromatic region of the ferro-cytochrome spectrum were investigated.

(PDF emailed within 1 workday: $29.90)

Accession: 017732437

Download citation: RISBibTeXText

PMID: 6087896

Related references

Electronic and vibrational spectroscopy of the cytochrome c:cytochrome c oxidase complexes from bovine and Paracoccus denitrificans. Protein Science 1(11): 1428-1434, 1992

Resonance Raman spectroscopy of the cytochrome c oxidase from Paracoccus denitrificans. Biochemistry 32(27): 6923-6927, 1993

ATR-FTIR Spectroscopy of the PM and F Intermediates of Bovine and Paracoccus denitrificans Cytochrome c Oxidase. Biochemistry (American Chemical Society) 42(29): 09-17, 2003

ATR-FTIR spectroscopy and isotope labeling of the PM intermediate of Paracoccus denitrificans cytochrome c oxidase. Biochemistry 43(45): 14370-8, 2004

Kinetics of the interaction of cytochrome c oxidase of Paracoccus denitrificans with Paracoccus and mitochondrial cytochrome c. Progress in Clinical and Biological Research 274: 619-635, 1988

Electrochemically driven respiration in mitochondria and Paracoccus denitrificans. The coupling of the electrochemistry of horse heart cytochrome c with respiration in mitochondria and a model thereof, Paracoccus denitrificans. Febs Letters 154(2): 319-322, 1983

Carboxyl group protonation upon reduction of the Paracoccus denitrificans cytochrome c oxidase: Direct evidence by FTIR spectroscopy. FEBS Letters 385(1-2): 53-57, 1996

Involvement of glutamic acid 278 in the redox reaction of the cytochrome c oxidase from Paracoccus denitrificans investigated by FTIR spectroscopy. Biochemistry 37(20): 7390-7399, 1998

Time-resolved ATR-FTIR spectroscopy of the oxygen reaction in the D124N mutant of cytochrome c oxidase from Paracoccus denitrificans. Biochemistry 46(45): 13141-8, 2007

Structural and chemical changes of the P(M) intermediate of paracoccus denitrificans cytochrome c oxidase revealed by IR spectroscopy with labeled tyrosines and histidine. Biochemistry 45(36): 10873-10885, 2006

Similarities and dissimilarities in the structure-function relation between the cytochrome c oxidase from bovine heart and from Paracoccus denitrificans as revealed by FT-IR difference spectroscopy. FEBS Letters 458(2): 83-86, Sept 17, 1999

Functional properties of the heme propionates in cytochrome c oxidase from Paracoccus denitrificans. Evidence from FTIR difference spectroscopy and site-directed mutagenesis. Biochemistry (American Chemical Society) 39(6): 56-63, 2000

Effects of mono clonal antibodies to beef heart and paracoccus denitrificans cytochrome c on reactions of cytochrome c with cytochrome oxidases reductases and peroxidase. Federation Proceedings 41(4): ABSTRACT 3662, 1982

Redox dependent changes at the heme propionates in cytochrome c oxidase from Paracoccus denitrificans: Direct evidence from FTIR difference spectroscopy in combination with heme propionate 13C labeling. Biochemistry 37(20): 7400-7406, May 19, 1998

Isolation of ubiquinol oxidase from Paracoccus denitrificans and resolution into cytochrome bc1 and cytochrome c-aa3 complexes. Journal of Biological Chemistry 260(4): 2458-2467, 1985