A putative H+-K+-ATPase in the Atlantic stingray, Dasyatis sabina: primary sequence and expression in gills
Choe, K.P.; Verlander, J.W.; Wingo, C.S.; Evans, D.H.
American Journal of Physiology. Regulatory Integrative and Comparative Physiology 287(4): R981-R991
ISSN/ISBN: 0363-6119 PMID: 15217793 DOI: 10.1152/ajpregu.00513.2003
In mammals, the gastric H(+)-K(+)-ATPase (HKalpha1) mediates acid secretion in the stomach and kidneys. Like mammals, elasmobranchs also secrete acid from their stomachs, but unlike mammals they primarily use their gills for systemic acid excretion instead of their kidneys. The purpose of this study was to determine if an HKalpha1 orthologue exists in an elasmobranch (Atlantic stingray, Dasyatis sabina), to determine if it is expressed in gills and, if so, to localize its expression and determine if its expression is regulated during hypercapnia or freshwater acclimation. A polyclonal antibody made against an HKalpha1 peptide detected HKalpha1 immunoreactivity in protein isolates and tissue sections of stingray stomachs and gills.