EurekaMag.com logo
+ Translate

Difference in the ability of neonatal and adult denervated muscle to accumulate acetylcholinesterase at the old sites of innervation


, : Difference in the ability of neonatal and adult denervated muscle to accumulate acetylcholinesterase at the old sites of innervation. Developmental Biology 117(1): 13-23

In adult rat sternocleidomastoid muscle, AChE is concentrated in the region rich in motor end-plates (MEP). All major AChE forms, “16 S,” “10 S,” and “4 S,” are accumulated at high levels, and not only “16 S” AChE. After denervation, muscle AChE decreases; 2 weeks after denervation, low levels (20–40% of control) are reached for all forms. During the following weeks, a slow but steady increase in “10 S” and “16 S” AChE occurs in the denervated muscle. At this stage, all forms are again observed to be highly concentrated in the region containing the old sites of innervation. Thus, in adult rat muscle the structures able to accumulate “16 S,” “10 S,” and “4 S” AChE in the MEP-rich regions remain several months after denervation. In normal young rat sternocleidomastoid muscle at birth, all AChE forms are already accumulated in the MEP-rich region. After denervation at birth, the denervated muscle loses its ability to keep a high concentration of “4 S,” “10 S,” and “16 S” AChE in the old MEP-rich region. All AChE forms are still present 1 month after denervation, but they are decreased and diffusedly distributed over the whole length of the muscle. In particular, “16 S” AChE is detected in the same proportion (10–15%) all along the denervated muscle. Thus, the diffuse distribution of AChE, and especially “16 S” AChE, after neonatal denervation, contrasts with the maintained accumulation observed in adult denervated muscle. It seems that denervation of young muscle results in a specific loss of the muscle ability to concentrate high levels of all AChE forms at the old sites of innervation.

(PDF same-day service: $19.90)

Accession: 017853942

PMID: 3743893

DOI: 10.1016/0012-1606(86)90343-x

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Blondet B.; Rieger F.; Gautron J.; Pincon Raymond M., 1986: Differences in the ability of neonatal and adult denervated muscle to accumulated acetylcholinesterase at the old sites of innervation. In adult rat sternocleidomastoid muscle, AChE is concentrated in the region rich in motor end-plates (MEP). All major AChE forms, "16 S", "10 S", and "4 S," are accumulated at high levels, and not only "16 S"...

Dennis, R.G.; Dow, D.E.; Hsueh, A.; Faulkner, J.A., 2002: Excitability of engineered muscle constructs, denervated and stimulated-denervated muscles of rats, and control skeletal muscles in neonatal, young, adult and old mice and rats. Biophysical Journal 82(1 Part 2): 364a, January

D.T.i N.A.; Vigny M.; Koenig H.L., 1984: Cytochemical and biochemical modifications of acetylcholinesterase and acetylcholinesterase receptors of denervated sternomastoid muscle of the trembler mouse. Biology of the Cell (Paris) 52(2): 97A

Maldonado, M.; Ramírez, B.U.; Ruiz, G.; Yamuy, J.; Inestrosa, N.C., 1984: Age-related responses of skeletal muscle after ectopic innervation, with particular reference to 16S acetylcholinesterase, in adult rats. The formation of ectopic junctions between the foreign fibular nerve and the soleus muscle of young (35-day-old) and mature (200-day-old) adult rats was induced by severing the normal nerve 4 weeks after implanting the foreign nerve. The various m...

Boudreau Lariviere, C.; Chan, R.Y.Y.; Mankal, F.A.; Jasmin, B.J., 1996: Innervation-independent transcriptional regulation of the acetylcholinesterase gene in adult skeletal muscle fibers. Society for Neuroscience Abstracts 22(1-3): 1101

Sadeh, M.; Stern, L.Z.; Czyzewski, K.; Finley, P.R.; Russell, D.H., 1984: Increased activities of MB and BB isozymes of creatine kinase in denervated neonatal and adult rat muscle. Creatine kinase (CK) activity and isozyme patterns were assessed in newborn and adult rat anterior tibial muscle in response to denervation. Total CK activity was low in the control neonatal muscle, gradually increasing to the adult level within 1...

Jay J.J.; Barald K.F., 1984: Distribution of acetylcholinesterase and alpha bungarotoxin on long term denervated adult rat muscle fibers in culture is unaffected by the presence of embryonic spinal cord explants. Society for Neuroscience Abstracts 10(2): 761

Kobayashi, T.; Askanas, V., 1985: Acetylcholine receptors and acetylcholinesterase accumulate at the nerve-muscle contacts of de novo grown human monolayer muscle cocultured with fetal rat spinal cord. Adult human biopsied muscle grown de novo from myoblasts in monolayer was cocultured with spinal cord explants of 13- to 14-day-old rat embryos. Cocultures were established 15 days after myoblast fusion. Autoradiography of 125I-alpha-bungarotoxin...

Connor E.A.; Rotshenker S.; Mcmahan U.J., 1986: Connective tissue cells that accumulate in the junctional region of denervated muscle are antigenically related to fibroblasts. Society for Neuroscience Abstracts 12(2): 1577

Kang, S-Bum.; Olson, J.L.; Atala, A.; Yoo, J.J., 2013: Functional recovery of completely denervated muscle: implications for innervation of tissue-engineered muscle. Tissue-engineered muscle has been proposed as a solution to repair volumetric muscle defects and to restore muscle function. To achieve functional recovery, engineered muscle tissue requires integration of the host nerve. In this study, we investi...