EurekaMag.com logo
+ Site Statistics
References:
53,623,987
Abstracts:
29,492,080
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Effect of adrenalectomy on Ca2+ signaling in rat hepatocytes



Effect of adrenalectomy on Ca2+ signaling in rat hepatocytes



Journal of Biological Chemistry 265(32): 19495-19501



To pursue our studies of the effects of adrenalectomy on the adrenergic regulation of phosphorylase a, cAMP, cell calcium, and Ca2+ signaling in rat hepatocytes (Studer, R.K., and Borle, A.B. (1984) Biochim. Biophys. Acta 804, 377-385; Freudenrich, C.C., and Borle, A.B. (1988) J. Biol. Chem. 263, 8604-8610), we have further examined the alpha 1-adrenergic pathway in adrenalectomized and sham-operated male rats. We measured the number and affinity of alpha 1-adrenergic receptors, the cytosolic free Ca2+ concentration [(Ca2+]i) of hepatocytes with aequorin, inositol triphosphate (IP3) accumulation, and Ca2+ influx and efflux across the plasma membrane. We also compared the effects of vasopressin with those obtained with epinephrine. We found that the number of alpha 1-adrenergic receptors was slightly depressed (-23%), but that their affinity was unchanged. However, IP3 accumulation evoked by epinephrine was decreased 50%. This is probably the main cause for the depressed peak rise in [Ca2+]i we previously observed and reported. We also found that the basal resting Ca2+ influx was increased after adrenalectomy. Experiments with the beta-blocker propranolol, which abolished the epinephrine-evoked increase in Ca2+ influx, suggest that this effect may be mediated by cAMP, at least in adrenalectomized animals. The effects of vasopressin on IP3 [Ca2+]i and Ca2+ influx and efflux were also significantly decreased after adrenalectomy, indicating that alpha 1-adrenergic-mediated and other IP3-dependent Ca2+ signaling pathways are depressed after adrenalectomy.

(PDF 0-2 workdays service: $29.90)

Accession: 017870541

Download citation: RISBibTeXText

PMID: 2174039



Related references

Effect of adrenalectomy on calcium signaling in rat hepatocytes. Journal of Biological Chemistry 265(32): 19495-19501, 1990

The effect of adrenalectomy on cytosolic free calcium in rat hepatocytes. Federation Proceedings 46(4): 1231, 1987

Na(+)-Ca2+ antiporter activity of rat hepatocytes. Effect of adrenalectomy on Ca2+ uptake and release from plasma membrane vesicles. Biochimica et Biophysica Acta 1134(1): 7-16, 1992

Effect of adrenalectomy and glucocorticoid treatment on the levels of an insulin-sensitive glycosyl-phosphatidylinositol in isolated rat hepatocytes. Molecular and Cellular Endocrinology 68(1): R1-R5, 1990

Effect of hydrocortisone adrenalectomy and their combination on the content of total lipids and phospholipids in the plasma membranes of rat hepatocytes. Farmakologiya i Toksikologiya (Moscow) 47(2): 46-49, 1984

Effect of adrenalectomy and dexamethasone treatment on the levels of an insulin sensitive glycosyl phosphatidylinositol in isolated rat hepatocytes. Diabetologia 32(7): 471A, 1989

Glucose and ketone body production in hepatocytes isolated from fetuses at term ii. effect of maternal adrenalectomy. Comparative Biochemistry and Physiology B 83(1): 231-234, 1986

Glucose and ketone bodies production in hepatocytes isolated from fetuses at term--II. Effect of maternal adrenalectomy. Comparative Biochemistry and Physiology. B, Comparative Biochemistry 83(1): 231-233, 1986

Effect of adrenalectomy on cellular calcium metabolism and the response to adrenergic stimulation of hepatocytes isolated from male and female rats. Biochimica et Biophysica Acta 804(3): 377-385, 1984

Sodium calcium antiporter activity of rat hepatocytes effect of adrenalectomy on calcium uptake and release from plasma membrane vesicles. Biochimica et Biophysica Acta 1134(1): 7-16, 1992

The effect of retroperitoneal fat mass on surgical outcomes in patients performing laparoscopic adrenalectomy: the effect of fat tissue in adrenalectomy. Surgical Innovation 17(2): 114-119, 2010

Effect of inhibiting vacuolar acidification on insulin signaling in hepatocytes. Journal of Biological Chemistry 279(13): 12777-12785, 2003

Effect of adrenalectomy and adrenalectomy plus hydrocortisone treatment on histopathological, biochemical and zinc and copper profiles in rat testes. Indian Journal of Experimental Biology 33(9): 655-663, 1995