+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Effect of cAMP on prostaglandin E2 production in cultured rat inner medullary collecting tubule cells



Effect of cAMP on prostaglandin E2 production in cultured rat inner medullary collecting tubule cells



American Journal of Physiology 251(4 Pt 2): F671-F677



Studies were performed to determine whether cAMP impairs prostaglandin (PG) E2 production in a homogeneous population of cultured rat inner medullary collecting duct cells. Three structurally different cAMP analogues were shown to decrease PGE2 synthesis by 48.4% in the basal state and by 49.3% in response to the divalent cation ionophore A23187 (5 microM). Thromboxane B2 production was similarly suppressed. An increase in endogenous cAMP by forskolin also decreased PGE2 synthesis. To determine the locus of the cAMP effect we examined the response to exogenously added arachidonic acid. At a concentration of arachidonic acid (5 micrograms/ml) sufficient to render the phospholipase-dependent fraction negligible (as evidenced by the lack of a mepacrine effect), cAMP had no effect on PGE2 production, suggesting phospholipase as the site of cAMP action. Further evidence for a phospholipase-mediated mechanism derives from studies employing [5,6,8,9,11,12,14,15-3H(N)]arachidonic acid in which cAMP analogues had no effect on the rate of cellular arachidonic acid incorporation, but did impair the release of tritiated arachidonic acid in response to ionophore. These results suggest the existence of a negative feedback system that, by impairing phospholipase activity and PGE2 synthesis, could enhance the action of cAMP in the antidiuretic state.

(PDF emailed within 1 workday: $29.90)

Accession: 017870920

Download citation: RISBibTeXText

PMID: 3020997


Related references

Effect of cyclic amp on prostaglandin e 2 production in cultured rat inner medullary collecting tubule cells. American Journal of Physiology 251(4 PART 2): F671-F677, 1986

Evidence for a role of calmodulin in cellular cAMP production in response to vasopressin, prostaglandin E2 and forskolin in cultured rat renal papillary collecting tubule cells. Nihon Naibunpi Gakkai Zasshi 61(8): 823-834, 1985

Effects of lithium on cAMP generation in cultured rat inner medullary collecting tubule cells. Kidney International 37(5): 1211-1218, 1990

Control of prostaglandin E2 synthesis in cultured rat inner medullary collecting tubule cells: the role of calcium. Mineral and Electrolyte Metabolism 12(5-6): 308-313, 1986

Control of vasopressin stimulated cyclic amp synthesis in cultured rat inner medullary collecting tubule cells roles of extracellular and intracellular calcium and prostaglandins prostaglandin e 2. Kidney International 27(1): 335, 1985

Myo-inositol uptake by rat cultured inner medullary collecting tubule cells: effect of osmolality. American Journal of Physiology 260(5 Pt 2): F619-F625, 1991

Effect of epinephrine on cAMP accumulation in cultured rat inner medullary collecting duct cells. American Journal of Physiology 272(2 Pt 2): F192-F197, 1997

Adrenergic control of cAMP generation in rat inner medullary collecting tubule cells. Kidney International 35(2): 647-653, 1989

Effect of cAMP agonists on cell pH and anion transport by cultured rat inner medullary collecting duct cells. American Journal of Physiology 270(1 Pt 2): F131-F140, 1996

Effect of nitric oxide on vasopressin dependent cAMP metabolism in cultured rat inner medullary collecting duct cells. Journal of the American Society of Nephrology 6(3): 325, 1995

Evidence for a role of calmodulin in cellular cyclic amp production in response to vasopressin prostaglandin e 2 and forskolin in cultured rat renal papillary collecting tubule cells. Folia Endocrinologica Japonica 61(8): 823-834, 1985

Endothelin 1 et 1 inhibits camp production by arginine vasopressin avp via pertussis toxin pt and staurosporine st sensitive pathway in cultured rat inner medullary collecting duct imcd cells. Journal of the American Society of Nephrology 3(3): 504, 1992

Control of prostaglandin e 2 synthesis by rat inner medullary collecting tubule cells role of cyclic amp. Kidney International 27(1): 253, 1985

Hormonal regulation of MAP kinase in cultured rat inner medullary collecting tubule cells. American Journal of Physiology 267(3 Pt 2): F366-F373, 1994

Cyclic amp impairs prostaglandin e 2 synthesis in rat inner medullary collecting tubule cells by a phospholipase independent mechanism. Clinical Research 33(2 PART 1): 500A, 1985