+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Effects of Na+ replacement and amiloride on ursodeoxycholic acid-stimulated choleresis and biliary bicarbonate secretion



Effects of Na+ replacement and amiloride on ursodeoxycholic acid-stimulated choleresis and biliary bicarbonate secretion



American Journal of Physiology 252(2 Pt 1): G163-G169



In these studies, we have tested the hypothesis that bile acid-dependent bile formation is attributable, in part, to the stimulation of active bicarbonate secretion and have further explored the cellular mechanism(s) possibly involved in this process using the isolated perfused rat liver. Under control conditions, ursodeoxycholic acid (UDCA) infusion (3 mumol/min X 20 min) produced a 3.7-fold increase in bile flow and a 7.4-fold increase in HCO3- output. Amiloride (an inhibitor of Na+-H+ exchange) decreased UDCA-stimulated bile flow by 20.6% and decreased biliary HCO3- output by 24.9% but increased biliary UDCA output by 42.9%. Thus amiloride decreased UDCA choleretic efficiency (microliter UDCA-stimulated bile/mumol UDCA output) by 45% and UDCA-stimulated increase in HCO3- output per unit UDCA secreted by 48%. Substitution of Li+ for Na+ in perfusate virtually abolished (greater than 95% decrease) both the UDCA choleresis and increase in biliary HCO3- output but modestly decreased (39.6%) biliary bile acid output. Li+ substitution thus decreased UDCA choleretic efficiency by 98% and the UDCA-stimulated increase in HCO3- output by 96%. Amiloride had no effect and Li+ substitution produced a modest decrease in basal bile flow (26.0%) and HCO-3 output (33.5%). Neither amiloride nor Li+ substitution significantly affected UDCA uptake by cultured hepatocytes or by perfused liver. Amiloride (1 mM) also decreased taurocholate (TC)-stimulated choleresis by 48.5%, biliary TC output by 7.2%, and the choleretic efficiency of TC by 45%.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 017875300

Download citation: RISBibTeXText

PMID: 3826345


Related references

Effects of sodium replacement and amiloride on ursodeoxycholic acid stimulated choleresis and biliary bicarbonate secretion. American Journal of Physiology 252(2 PART 1): G163-G169, 1987

Ursodeoxycholic acid stimulated choleresis and bicarbonate secretion are sodium dependent processes. Gastroenterology 88(5 PART 2): 1673, 1985

Ursodeoxycholic acid choleresis relationship to biliary bicarbonate secretion and further evidence of a role for sodium proton exchange. Hepatology 5(5): 1011, 1985

Ursodeoxycholic acid choleresis relationship to biliary bicarbonate and effects of sodium proton exchange inhibitors. American Journal of Physiology 254(2 PART 1): G232-G241, 1988

Inhibition of ursodeoxycholic acid induced hypercholeresis by sodium replacement or amiloride is associated with replacement of unconjugated ursodeoxycholic acid by ursodeoxycholic acid glucuronides in bile. Hepatology 5(5): 1010, 1985

Ursodeoxycholic acid choleresis: relationship to biliary HCO-3 and effects of Na+-H+ exchange inhibitors. American Journal of Physiology 254(2 Pt 1): G232-G241, 1988

Ursodeoxycholic acid induced biliary bicarbonate secretion is inhibited by heavy water. Journal of Hepatology 11(SUPPL 2): S30, 1990

Effects of inhibitors of carbonic anhydrase and amiloride on biliary bicarbonate secretion. Gastroenterologie Clinique et Biologique 10(1): 68-69, 1986

Ursodeoxycholic Acid Ameliorates Intrahepatic Cholestasis Independent of Biliary Bicarbonate Secretion in Vil2 kd/kd Mice. Biological and Pharmaceutical Bulletin 40(1): 34-42, 2017

Mechanism of ursodeoxycholic acid- and canrenoate-induced biliary bicarbonate secretion and the effect on glucose- and amino acid-induced cholestasis. Scandinavian Journal of Gastroenterology 26(5): 513-522, 1991

Effects of ursodeoxycholic acid on the biliary lipids secretion. Nihon Yakurigaku Zasshi. Folia Pharmacologica Japonica 78(4): 261-267, 1981

Effects of bile acid depletion and of ursodeoxycholic acid udc and chenodeoxycholic acid cdc on biliary protein p secretion. Gastroenterology 96(5 PART 2): A626, 1989

Effect of carbonic anhydrase inhibitors and amiloride on ursodeoxycholate induced biliary bicarbonate secretion. Hepatology 5(5): 982, 1985

Effect of simvastatin ursodeoxycholic acid and simvastatin plus ursodeoxycholic acid on biliary lipid secretion and cholic acid kinetics in nonfamilial hypercholesterolemia. Hepatology 15(6): 1072-1078, 1992

Effect of ursodeoxycholic acid on biliary lipid secretion and bile acid metabolism in primary biliary cirrhosis. Hepatology 8(5): 1317, 1988