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LiCl treatment releases a nickase implicated in genetic transformation of Streptococcus pneumoniae



LiCl treatment releases a nickase implicated in genetic transformation of Streptococcus pneumoniae



Journal of Bacteriology 169(11): 4901-4906



When cells competent for genetic transformation of Streptococcus pneumoniae which could bind and enable entry of extracellular DNA molecules were treated with LiCl, they released a nickase that introduced nicks into a double-stranded DNA in the presence of EDTA. The nickase was specific for competent cells and coupled with DNA-binding activity. Furthermore, when noncompetent cells were treated with LiCl, they released the putative receptors for the competence activator.

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Accession: 017985466

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PMID: 2822653


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