+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

New U1 RNA species found in Friend SFFV (spleen focus forming virus)-transformed mouse cells

New U1 RNA species found in Friend SFFV (spleen focus forming virus)-transformed mouse cells

Journal of Biological Chemistry 260(12): 7775-7782

The U1 RNA species in 10 mouse cell lines were examined by two-dimensional polyacrylamide gel electrophoresis. Seven cell lines that were not infected by Friend spleen focus forming virus gave only one (I) or two (I and II) U1 RNA-containing spots. However, two Friend cell lines (FVTCT and Friend 745a cells) gave three spots (I, II, and III) and another Friend cell line, K-1 cells, gave four spots (I, II, III, and IV). As a result of further separation and fingerprinting analysis of each spot, FVTCT and Friend 745a cells were found to contain U1a-1, U1b-1, -2, and -6 RNAs whereas K-1 cells were found to contain several U1 RNAs, which we call U1a-1 and -2, U1b-4, -5, and -6 RNAs. We determined the sequences of these seven U1 RNAs and found that mouse U1 RNAs had two basic sequences (U1a and -b). The nucleotide sequence of U1a-1 RNA was identical to that of rat U1a RNA, while U1a-2 RNA was one base different from U1a-1 RNA. Relative to U1a-1 RNA all of the U1b RNAs had five base substitutions and one additional base and were under-methylated in the center. U1b-6 RNA contained two base substitutions and one base addition in the 3'-terminal portion of U1b-1 RNA. U1b-2, -4, and -5 RNAs, which were observed only in Friend cells, each had an additional base substitution in the 5'-half of U1b-1 RNA.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 018027082

Download citation: RISBibTeXText

PMID: 2581971

Related references

A 585-bp deletion found in the spleen focus-forming virus (SFFV) env gene is responsible for the defective intracellular transport of SFFV gp52. Virology 188(1): 181-192, 1992

Structural and functional studies of the Friend spleen focus-forming virus: structural relationship of SFFV to dualtropic viruses and molecular cloning of a biologically active subgenomic fragment of SFFV DNA. Haematology and Blood Transfusion 26: 472-478, 1981

A new series of RNAs associated with the genome of spleen focus forming virus (SFFV) and poly(A)-containing RNA from SFFV-infected cells. Nucleic Acids Research 7(4): 895-908, 1979

Oligomerization of Friend spleen focus-forming virus (SFFV) env glycoproteins. Virology 177(1): 312-316, 1990

Identification of Proteins Specific to Friend Strain of Spleen Focus forming Virus (SFFV). Proceedings of the Japan Academy Series B Physical and Biological Sciences 54(10): 651-656, 1978

B-tropic Friend virus: a host-range pseudotype of spleen focus-forming virus (SFFV). Virology 55(2): 363-370, 1973

Induction of endogenous and of spleen focus-forming viruses during dimethylsulfoxide-induced differentiation of mouse erythroleukemia cells transformed by spleen focus-forming virus. Proceedings of the National Academy of Sciences of the United States of America 72(5): 1863-1867, 1975

Heteroduplex analysis of molecular clones of the pathogenic Friend virus complex: Friend murine leukemia virus, Friend mink cell focus-forming virus, and the polycythemia- and anemia-inducing strains of Friend spleen focus-forming virus. Journal of Virology 51(2): 306-314, 1984

Molecular analysis of the cell type specific mitogenic activity of the env like glycoprotein gp55 of friend spleen focus forming virus sffv. Nishizuka, Y , M Endo And C Tanaka (Ed ) Advances in Second Messenger And Phosphoprotein Research, Vol 24 The Biology And Medicine Of Signal Transduction; 7th International Conference on Cyclic Nucleotides, Calcium And Protein Phosphorylation, Kobe, Japan, October 8-13, 1989 Xxxiii+750p Raven Press: New York, New York, Usa Illus 702, 1990

Mutational analysis of the structure-function relationship of the leukemogenic membrane glycoprotein (GP55) of Friend spleen focus-forming virus (F-SFFV). Leukemia 11(Suppl. 3): 160-161, 1997

The spleen focus-forming virus (SFFV) envelope gene, when introduced into mice in the absence of other SFFV genes, induces acute erythroleukemia. Journal of Virology 62(6): 2158-2163, 1988

Immunological analysis of specific glyco protein gp 55 in cells transformed by the friend strain of spleen focus forming virus. Bulletin of the Nippon Veterinary & Animal Science University (31): 156-163, 1982

Multipotential hemopoietic cell lines isolated from stem cell cultures infected with friend virus complex murine leukemia virus plus friend spleen focus forming virus show presence of murine leukemia virus but not friend spleen focus forming virus. Leukemia Research 10(2): 187-194, 1986

A new series of Rnas associated with the genome of spleen focus fonning virus (Sffv) and poly(A)-containing Rna from Sffv-infected cells. Nucleic Acids Research 7(4): 895-908, 1979

Fv 2 r mediated resistance of mouse bone marrow cells to friend spleen focus forming virus infection. International Journal of Cancer 26(1): 101-106, 1980