+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Primary hepatocytes in monolayer culture: a model for studies on lipoprotein metabolism



Primary hepatocytes in monolayer culture: a model for studies on lipoprotein metabolism



Annual Review of Physiology 46: 403-415



Primary hepatocyte cultures are ideal models in which to investigate regulation of lipoprotein synthesis and catabolism by the liver. Not only can one regulate the physiological and nutritional state of the donor animal, but one can also manipulate the culture milieu. A possible problem that arises in prolonged incubations is the accumulation of metabolites in the culture media, which may alter the function of the cells.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 018066430

Download citation: RISBibTeXText

PMID: 6370114

DOI: 10.1146/annurev.ph.46.030184.002155


Related references

Drug metabolism in adult rat hepatocytes in primary monolayer culture. Gastroenterology 72(6): 1232-1239, 1977

Species differences in the metabolism of 2-acetylaminofluorene by hepatocytes in primary monolayer culture. Cancer Research 46(4 Pt 1): 1627-1632, 1986

Monolayer culture of primary rat hepatocytes on an Arg-Gly-Asp RGD-immobilized polystyrene dish express liver-specific functions of albumin production and p-acetamidophenol metabolism the same as for spheroid culture. Biochemical Engineering Journal 40(2): 387-391, 2008

Effects of insulin on protein metabolism in adult rat hepatocytes in primary monolayer culture. Horumon to Rinsho. Clinical Endocrinology 29(9): 1115-1122, 1981

Growth control of differentiated fetal rat hepatocytes in primary monolayer culture. IX. Specific inhibition of DNA synthesis initiation by very low density lipoprotein and possible significance to the problem of liver regeneration. Journal of Cell Biology 70(1): 20-32, 1976

Growth Control of Differentiated Fetal Rat Hepatocytes in Primary Monolayer Culture. Ix. Specific Inhibition of Dna Synthesis Initiation by Very Low Density Lipoprotein and Possible Significance to the Problem of Liver Regeneration. The Journal of Cell Biology 70(1): 20-32, 1976

Primary monolayer culture of adult mouse hepatocytes -- a model for the study of hepatotropic viruses. Archives of Virology 63(1): 11-22, 1980

Hepatocytes in monolayer culture an in vitro model for toxicity studies. Mcqueen, C A (Ed ) in Vitro Toxicology: Model Systems And Methods Vii+292p Telford Press: Caldwell, New Jersey, Usa Illus 131-156, 1989

Effects of preincubation of primary monolayer cultures of rat hepatocytes with low- and high-density lipoproteins on the subsequent binding and metabolism of human low-density lipoprotein. Biochemical Journal 247(1): 79-84, 1987

Human adult hepatocytes in primary monolayer culture. Maintenance of mixed function oxidase and conjugation pathways of drug metabolism. Biochemical Pharmacology 36(14): 2311-2316, 1987

Studies with mature rat hepatocytes in monolayer culture. I. Propagation of isolated hepatocytes, morphological and biochemical in culture. Cell and Tissue Research 169(2): 167-178, 1976

Suitability of primary monolayer cultures of adult rat hepatocytes for studies of cholesterol and bile acid metabolism. Journal of Biological Chemistry 260(2): 1015-1019, 1985

Bile acid synthesis and secretion by rabbit hepatocytes in primary monolayer culture comparison with rat hepatocytes. Biochimica et Biophysica Acta 1001(2): 176-184, 1989

Effect of cobalt on synthesis of heme and cytochrome P-450 in the liver. Studies of adult rat hepatocytes in primary monolayer culture and in vivo. Journal of Biological Chemistry 251(14): 4421-4427, 1976

Growth Control Of Differentiated Fetal Rat Hepatocytes In Primary Monolayer Culture: Vi. Studies with Conditioned Medium and Its Functional Interactions with Serum Factors. The Journal of Cell Biology 62(3): 780-791, 1974