EurekaMag
+ Translate
+ Most Popular
Advantages and disadvantages of bordeaux mixture and of lime-sulphur used on apples in the growing season
Observations on the Umaria marine bed
10 years of hearing conservation in the Royal Air Force
Chocolate crumb - dairy ingredient for milk chocolate
Effect of daily gelatin ingestion on human scalp hair
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
The composition of pampas-grass (Cortaderia argentea.)
The Accraian Series:
The mechanism of the Liebermann-Burchard reaction of sterols and triterpenes and their esters
Cerebrovascular Doppler ultrasound studies (cv-Doppler)
Toria: PT-303 - first national variety
Hair growth promoting activity of tridax procumbens
Productivity of Pekin x Khaki Campbell ducks
A stable cytosolic expression of VH antibody fragment directed against PVY NIa protein in transgenic potato plant confers partial protection against the virus
Solar treatment of wheat loose smut
Swimmers itch in the Lake of Garda
Bactofugation and the Bactotherm process
The effects of prefrontal lobotomy on aggressive behavior in dogs
Visual rating scales for screening whorl-stage corn for resistance to fall armyworm
Breakdown of seamounts at the trench axis, viewed from gravity anomaly
Kooken; pennsylvania's toughest cave
Recovery of new dinosaur and other fossils from the Early Cretaceous Arundel Clay facies (Potomac Group) of central Maryland, U.S.A
Zubor horny (Bison bonasus) v prirodnych podmienkach Slovensku
The extended Widal test in the diagnosis of fevers due to Salmonella infection
Hair of the american mastodon indicates an adaptation to a semi aquatic habitat

Purification, characterization, and amino acid sequence of an embryonic lectin in perivitelline fluid of the horseshoe crab


Purification, characterization, and amino acid sequence of an embryonic lectin in perivitelline fluid of the horseshoe crab



Journal of Biological Chemistry 274(53): 37673-37678



ISSN/ISBN: 0021-9258

PMID: 10608824

DOI: 10.1074/jbc.274.53.37673

Hemagglutinating activity in perivitelline fluid of the horseshoe crab embryo dramatically increases during the third and fourth molt of the embryo. A 27-kDa lectin, which we named tachylectin-P (TL-P), was newly identified in perivitelline fluid of the horseshoe crab Tachypleus tridentatus. TL-P preferentially agglutinated human A-type erythrocytes, and the activity was inhibited by N-acetyl group-containing monosaccharides. The amino acid sequence analysis indicated that TL-P is almost structurally the same as a hemocyte-derived lectin with no hemagglutinating activity, tachylectin-1 (TL-1), and that 218 out of 221 amino acid residues in total were conserved between the two lectins. Despite the high sequence similarity, biological and biochemical characteristics of TL-P differed from those of TL-1: (i) unlike TL-1, TL-P agglutinates several animal-derived erythrocytes; (ii) unlike TL-1, TL-P has no significant affinity for bacterial lipopolysaccharides or antibacterial activity; (iii) Based on apparent molecular masses determined by gel filtration, TL-P forms a dimer in solution, while TL-1 is present as a monomer; (iv) and TL-P interacts with endogenous proteins of 13 and 14 kDa present in the perivitelline fluid; however, neither has any affinity for TL-1. We propose that TL-P may have an important role in completing embryonic development by interacting with endogenous glycoproteins or N-acetylhexosamines.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 018077199

Download citation: RISBibTeXText

Related references

Studies on Perivitelline Fluid of Horseshoe Crab Embryo. Ii. Purification of Agglutinin-Binding Substance from the Perivitelline Fluid of Tachypleus gigas Embryo12. The Journal of Biochemistry 96(3): 629-636, 1984

Studies on perivitelline fluid of horseshoe crab embryo. II. Purification of agglutinin-binding substance from the perivitelline fluid of Tachypleus gigas embryo. Journal of Biochemistry 96(3): 629-636, 1984

Studies on perivitelline fluid of horseshoe crab embryo. I. Purification and properties of agglutinin from the perivitelline fluid of Tachypleus gigas embryo. Journal of Biochemistry 96(3): 621-628, 1984

Enhancement of vertebrate cardiogenesis by a lectin from perivitelline fluid of horseshoe crab embryo. Cellular and Molecular Life Sciences: Cmls 65(20): 3312-3324, 2008

Enhancement of angiogenesis by a 27 kDa lectin from perivitelline fluid of horseshoe crab embryos through upregulation of VEGF and its receptor. Journal of Natural Products 76(1): 117-120, 2013

Purification and amino acid sequence of Kunitz-type protease inhibitor found in the hemocytes of horseshoe crab (Tachypleus tridentatus). Journal of Biochemistry 101(5): 1297-1306, 1987

Purification, characterization, and cDNA cloning of a 27-kDa lectin (L10) from horseshoe crab hemocytes. Journal of Biological Chemistry 270(52): 31008-31015, 1995

Isolation, purification and characterization of a lectin from the Chinese horseshoe crab, Tachypleus tridentatus. Chinese Biochemical Journal 66: 505-510, 1990

On a thin membrane formed in the perivitelline space during the early embryonic development of the horseshoe crab. Acta Arachnol Tokyo 271: 31-36, 1976

Changes in Osmotic Pressure and Swelling in Horseshoe Crab Embryos During Development : (horseshoe crab embryo/swelling/water influx/perivitelline fluid/osmotic pressure). Development, Growth and Differentiation 27(1): 51-56, 1985

Protein components in the perivitelline fluid of the embryo of the horseshoe crab,Tachypleus tridentatus. Developmental Biology 73(2): 183-192, 1979

Effects of Perivitelline Fluid Obtained from Horseshoe Crab on The Proliferation and Genotoxicity of Dental Pulp Stem Cells. Cell Journal 17(2): 253-263, 2015

N-terminal amino acid sequence comparison of Asian horseshoe crab hemocyanins: Immunologically identical hemocyanin subunits are orthologous in Asian horseshoe crabs. Zoological Science 15(2): 295-299, 1998

Further characterization of the sialic acid-binding lectin from the horseshoe crab Carcinoscorpius rotunda cauda. Archives of Biochemistry and Biophysics 209(1): 325-333, 1981

Molecular characterization of limulin, a sialic acid binding lectin from the hemolymph of the horseshoe crab, Limulus polyphemus. Biochemistry 16(19): 4297-4303, 1977