EurekaMag
+ Translate
+ Most Popular
Advantages and disadvantages of bordeaux mixture and of lime-sulphur used on apples in the growing season
Observations on the Umaria marine bed
10 years of hearing conservation in the Royal Air Force
Chocolate crumb - dairy ingredient for milk chocolate
Effect of daily gelatin ingestion on human scalp hair
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
The composition of pampas-grass (Cortaderia argentea.)
The Accraian Series:
The mechanism of the Liebermann-Burchard reaction of sterols and triterpenes and their esters
Cerebrovascular Doppler ultrasound studies (cv-Doppler)
Toria: PT-303 - first national variety
Hair growth promoting activity of tridax procumbens
Productivity of Pekin x Khaki Campbell ducks
A stable cytosolic expression of VH antibody fragment directed against PVY NIa protein in transgenic potato plant confers partial protection against the virus
Solar treatment of wheat loose smut
Swimmers itch in the Lake of Garda
Bactofugation and the Bactotherm process
The effects of prefrontal lobotomy on aggressive behavior in dogs
Visual rating scales for screening whorl-stage corn for resistance to fall armyworm
Breakdown of seamounts at the trench axis, viewed from gravity anomaly
Kooken; pennsylvania's toughest cave
Recovery of new dinosaur and other fossils from the Early Cretaceous Arundel Clay facies (Potomac Group) of central Maryland, U.S.A
Zubor horny (Bison bonasus) v prirodnych podmienkach Slovensku
The extended Widal test in the diagnosis of fevers due to Salmonella infection
Hair of the american mastodon indicates an adaptation to a semi aquatic habitat

Rapid changes in tubulin RNA synthesis and stability induced by deflagellation in Chlamydomonas


Rapid changes in tubulin RNA synthesis and stability induced by deflagellation in Chlamydomonas



Journal of Cell Biology 99(6): 2074-2081



ISSN/ISBN: 0021-9525

PMID: 6209288

DOI: 10.2307/1610972

Detachment of the flagella of Chlamydomonas induces a rapid accumulation of mRNAs for tubulin and other flagellar proteins. Measurement of the rate of alpha and beta tubulin RNA synthesis during flagellar regeneration shows that deflagellation elicits a rapid, 4-7-fold burst in tubulin RNA synthesis. The synthesis rate peaks within 10-15 min, then declines back to the predeflagellation rate. Redeflagellation of cells at times before the first flagellar regeneration is completed (and when cells have already accumulated elevated levels of tubulin RNA) induces another burst in tubulin RNA synthesis which is identical to the first in magnitude and duration. This finding indicates that the induction signal may act to simply reprogram the tubulin genes for a transient burst of maximal synthesis. Evidence is presented that the stability of the tubulin RNAs changes during regeneration. Stability changes include both an apparent stabilization during regeneration and accelerated decay following regeneration.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 018081516

Download citation: RISBibTeXText

Related references

Protein synthesis is required for rapid degradation of tubulin mRNA and other deflagellation-induced RNAs in Chlamydomonas reinhardi. Molecular and Cellular Biology 6(1): 54-61, 1986

Multiple alpha- and beta-tubulin genes in Chlamydomonas and regulation of tubulin mRNA levels after deflagellation. Cell 24(1): 81-88, 1981

Mapping of regulatory elements responsible for induction of the alpha-1 tubulin gene after deflagellation of Chlamydomonas reinhardtii. Molecular Biology of the Cell 4(Suppl. ): 415A, 1993

Sequences controlling transcription of the Chlamydomonas reinhardtii beta 2-tubulin gene after deflagellation and during the cell cycle. Molecular and Cellular Biology 14(8): 5165-5174, 1994

Deflagellation of Chlamydomonas reinhardtii follows a rapid transitory accumulation of inositol 1,4,5-trisphosphate and requires Ca2+ entry. Journal of cell biology 123(4): 869-875, 1993

Deflagellation of Chlamydomonas reinhardtii Follows a Rapid Transitory Accumulation of Inositol 1,4,5,-Trisphosphate and Requires Ca2+Entry. The Journal of Cell Biology 123(4): 869-875, 1993

Deflagellation induced gene regulation in Chlamydomonas reinhardtii. Molecular Biology of the Cell 3(Suppl. ): 169A, 1992

Millisecond accumulation of Ins-1,4,5-P-3 preceeds acid induced Chlamydomonas reinhardtii deflagellation. Plant Physiology (Rockville) 102(1 Suppl. ): 116, 1993

Isolation and characterization of Chlamydomonas reinhardtii mutants with defects in induction of flagellar protein synthesis after deflagellation. Journal of Protozoology 354: 559-564, 1988

Isolation and characterization of Chlamydomonas reinhardtii mutants with defects in the induction of flagellar protein synthesis after deflagellation. Journal of Protozoology 35(4): 559-564, 1988

An intronic enhancer is required for deflagellation-induced transcriptional regulation of a Chlamydomonas reinhardtii dynein gene. Molecular Biology of the Cell 9(11): 3085-3094, 1998

Tubulin induction in chlamydomonas reinhardii requirement for tubulin messenger rna synthesis. Cell 24(1): 89-96, 1981

Tubulin gene expression in the Chlamydomonas reinhardtii cell cycle: elimination of environmentally induced artifacts and the measurement of tubulin mRNA levels. Journal of Cell Science 89: 397-403, 1988

Tubulin gene expression in the chlamydomonas reinhardtii cell cycle elimination of environmentally induced artifacts and the measurement of tubulin messenger rna levels. Journal of Cell Science 89(3): 397-404, 1988

Genetics of the deflagellation pathway in Chlamydomonas. Genetics 149(2): 927-936, 1998