+ Translate
+ Most Popular
Sympathetic and visceral nerve blocks
What should be done after bee or wasp stings?
Anti-drool cheiloplasty: clinical results in six dogs
The biology of human starvation: some new insights
Vitamin B12 deficiency as a worldwide problem
Effect of daily gelatin ingestion on human scalp hair
On the nature and role of vivotoxins in plant disease
History of the discovery of vitamins by N. I. Lunin
Preliminary tests with residual sprays against poultry lice
MDU.1, a new mutant chilli variety
A study on compatibility of some insecticides with a fungicide on small cardamom (Elettaria cardamomum Maton.)
The cranial nerves of Mystus seenghala (Sykes)
The effects of prefrontal lobotomy on aggressive behavior in dogs
Human monkeypox: disease pattern, incidence and attack rates in a rural area of northern Zaire
The identity of the lipstick mold of cultivated mushrooms agaricus bisporus
Therapy for acne with saccharomyces boulardii
Advantages and disadvantages of bordeaux mixture and of lime-sulphur used on apples in the growing season
The composition of pampas-grass (Cortaderia argentea.)
Productivity of Pekin x Khaki Campbell ducks
A rapid survey of katydids (Insecta: Orthoptera: Tettigoniidae) of the Foret Classee du Pic de Fon, Guinea
Cranial nerves of Labeo rohita (Ham.)
Evaluating fundamentals of care: The development of a unit-level quality measurement and improvement programme
Life-cycle of the free-living nematode Pontonema vulgare in the White Sea
Grey leaf blight - a new disease of cocoa
Effect of foliar spray of urea and zinc sulphate on yield and fruit quality of Kinnow - 'a mandarin hybrid'

Reaction of DNA-bound Co(II)bleomycin with dioxygen

Reaction of DNA-bound Co(II)bleomycin with dioxygen

Journal of Biological Chemistry 267(2): 950-955

ISSN/ISBN: 0021-9258

PMID: 1370471

The aerobic oxidation of Co(II)bleomycin bound to calf thymus DNA has been investigated in relation to the mechanism of reaction in solution in the absence of DNA. Kinetics of dioxygenation of the Co(II) complex were followed by spectrophotometric and electron spin resonance spectroscopy as well as dioxygen analysis. The reaction is slower than when carried out in solution; its rate is inversely related to the ratio of DNA base pairs to Co(II)bleomycin. The subsequent oxidation reaction, observed spectrophotometrically and by dioxygen analysis, is second order in cobalt complex. The calculated second order rate constant is also inversely related to the base pair to metal complex ratio. Once this ratio exceeds three, the reaction rate slows significantly with each additional increment of DNA added to the starting reaction mixture. Taking advantage of the high stability of O(2)-Co(II)bleomycin bound to greater than a 3-fold excess of DNA base pairs, it could be demonstrated that the rate constant for oxidation of two O(2)-Co(II)bleomycin molecules is much slower than that for O(2)-Co(II)bleomycin plus Co(II)bleomycin. With the same technique it was observed that the metal centers of O(2)-Co(II)bleomycin and Fe(II)bleomycin also undergo oxidation. The binding to DNA of both solution products of the oxidation of Co(II)bleomycin by O2 was examined by 1H NMR spectroscopy. Peroxy-Co(III)bleomycin, Form I, binds with higher affinity than Co(III)bleomycin, Form II. At lower ionic strength, the size of the DNA binding site for each form is about 2 base pairs/molecule of drug.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 018082651

Download citation: RISBibTeXText

Related references

Reaction of DNA-bound ferrous bleomycin with dioxygen: Activation versus stabilization of dioxygen. Biochemistry 33(17): 5319-5327, 1994

Orientation of dioxygen bound to cobalt(II) bleomycin-DNA fibers. Journal of Biological Chemistry 264(36): 21478-21480, 1989

Reaction of Co(II)bleomycin with dioxygen. Journal of Biological Chemistry 267(2): 944-949, 1992

Man designed bleomycins synthesis of dioxygen activating molecules and a dna cleaving molecule based on bleomycin iron ii dioxygen complex. Tetrahedron 44(10): 2821-2834, 1988

NMR determination of the structures of peroxycobalt bleomycin and Cobalt bleomycin, products of the aerobic oxidation of Cobalt bleomycin by dioxygen. Biochemistry 33(4): 907-916, 1994

NMR determination of the structures of peroxycobalt(III) bleomycin and cobalt(III) bleomycin, products of the aerobic oxidation of cobalt(II) bleomycin by dioxygen. Biochemistry 33(4): 907-916, 1994

Effect of the carbamoyl group attached to an axial ligand portion of a novel bleomycin model on a dioxygen activating reaction. Chemical and Pharmaceutical Bulletin 46(10): 1635-1638, 1998

Reaction of nitric oxide with iron bleomycin bound to DNA: properties of the nitrosyl adduct and its reaction with O2. Inorganica Chimica Acta 240(1-2): 535-540, 1995

Transition-Metal Binding Site of Bleomycin. A Synthetic Analogue Superior to Bleomycin in Dioxygen-Activating Capability 1. Synlett 1989(1): 70-71, 1989

Transition metal binding site of bleomycin a synthetic analogue superior to bleomycin in dioxygen activating capability. Synlett 1: 70-71, 1989

Transition metal binding site of bleomycin a remarkably efficient dioxygen activating molecule based on bleomycin iron ii complex. Tetrahedron Letters 27(31): 3631-3634, 1986

The 1.6-A crystal structure of the copper(II)-bound bleomycin complexed with the bleomycin-binding protein from bleomycin-producing Streptomyces verticillus. Journal of Biological Chemistry 277(3): 2311-2320, 2001

Reaction of the Escherichia coli quinol oxidase cytochrome bo3 with dioxygen: the role of a bound ubiquinone molecule. Proceedings of the National Academy of Sciences of the United States of America 93(4): 1545-1548, 1996

A novel mixed valence form of Rhus vernicifera laccase and its reaction with dioxygen to give a peroxide intermediate bound to the trinuclear center. Journal of Biochemistry 129(6): 949-953, 2001

Synthesis and characterization of alkanethiolate-coordinated iron porphyrins and their dioxygen adducts as models for the active center of cytochrome p450: direct evidence for hydrogen bonding to bound dioxygen. Journal of the American Chemical Society 123(6): 1133-1142, 2001